Replication of DNA Flashcards
Semiconservative Replication
parental strands of DNA separate, serve as templates, and produce DNA molecules that have on strand of parental DNA and one strand of new DNA
who suggested a possible method of replication when they presented their model of DNA to science cummunity
Watson and Crick
DNA helicase
an enzyme that is responsible for unwinding and unzipping the double helix
What happens when double helix is unzipped
Hydrogen bonds between the bases are broken, and leave single strands of DNA
What happens after there are single strand of DNA
proteins called single-stranded binding proteins associate with DNA to keep strands separate during replication
What happens after proteins associate with DNA to keep the strands separate
RNA primate adds a segment of RNA on each DNA strand
DNA polymerase
an enzyme that catalyze the addition of appropriate nucleotides to the new DNA strand
Where are the nucleotides added to after DNA polymerase
3’ at the end of new strand
Leading strand
elongated as the DNA unwinds - built continuously by the addition of nucleotides to the 3’ end
Lagging Strand
Elongates away from the replication fork - synthesized discontinuously into small segments (Okazaki fragments)
What are Okazaki fragments later connected by
enzyme DNA ligase
What happens when the DNA polymerase comes to an RNA primer on the DNA
it removed the primers and fills in the place with DNA nucleotides - when RNA primer has been replaced DNA ligase links the two sections
Prokaryote DNA is where
cytoplasm
Where is eukaryote DNA
nucleus
