Replication Flashcards

1
Q

What is the process of making an identical copy of DNA called?

A

Replication

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2
Q

Which enzyme unwinds the DNA double helix during replication?

A

Helicase

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3
Q

What is the function of DNA polymerase during replication?

A

Synthesizes new DNA strands

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4
Q

In which direction does DNA replication occur?

A

5’ to 3’

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5
Q

What is the role of primase in DNA replication?

A

Synthesizes RNA primers

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6
Q

What is the function of DNA ligase in DNA replication?

A

Joins Okazaki fragments

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7
Q

What is the role of single-strand binding proteins in DNA replication?

A

Prevents reannealing of DNA strands

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8
Q

What is the name of the DNA replication process where both strands are synthesized simultaneously?

A

Semi-conservative replication

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9
Q

Which enzyme proofreads newly synthesized DNA strands during replication?

A

DNA polymerase III

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10
Q

What is the function of topoisomerase during DNA replication?

A

Relieves strain in the DNA ahead of the replication fork

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11
Q

What is the term for the short DNA fragments formed on the lagging strand during replication?

A

Okazaki fragments

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12
Q

Which enzyme removes RNA primers and replaces them with DNA nucleotides during replication?

A

DNA polymerase I

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13
Q

What is the function of the sliding clamp during DNA replication?

A

Keeps DNA polymerase attached to the template strand

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14
Q

What is the name of the site where DNA replication begins?

A

Origin of replication

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15
Q

What is the role of the replisome in DNA replication?

A

Complex of enzymes involved in replication

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16
Q

What is the term for the process of separating the two DNA strands during replication?

A

DNA unwinding

17
Q

Which enzyme synthesizes the RNA primers needed for DNA replication?

A

Primase

18
Q

What is the name of the DNA sequence where DNA replication begins?

A

Replication origin

19
Q

What is the role of DNA ligase in the process of DNA replication?

A

Joins Okazaki fragments on the lagging strand

20
Q

What is the term for the region where DNA replication is occurring?

A

Replication fork

21
Q

Which direction does DNA polymerase synthesizes the new DNA strand?

A

5’ to 3’

22
Q

What is the name of the enzyme that joins the Okazaki fragments on the lagging strand during DNA replication?

A

DNA ligase

23
Q

What is the function of DNA gyrase during DNA replication?

A

Relieves supercoiling ahead of the replication fork

24
Q

Which enzyme is responsible for separating the DNA strands during replication?

A

Helicase