Ref Ranges, Formulas, Mnemonics Flashcards
Formula for Absorbance
2 - log%T
NOTE: The relationship between absorbance and %T is LOGARITHMIC
Formula for Absorbance under Beer-lambert’s law
A = εbc
ε = molar absorptivity (L/ mol x cm)
b = light path (cm)
c = concentration of analyte (mol/L)
Units for Molar Absorptivity
L/(mol x cm)
Ref: Serum Osm
280 - 300 mmol/kg
Ref: Osmol Gap
<10 mmol/kg
Formula for Osmolal Gap
Osmolal gap (mOsm/kg) = Measured osmolality (mOsm/kg) - Calculated osmolality (mmol/L)
Formula for Calculated Osmolality
[2Na+] + [glucose] + [urea] = mmol/L
Ref: pH
7.35 - 7.45
Ref: pCO2
35 - 45 mm Hg
Ref: pO2
70 - 90 mm Hg
Ref: HCO3-
20 -27 mmol/L
Ref: Sodium
133 - 146 mmol/L
Sodium Crit
<125 OR >155 mmol/L
Ref: Potassium
3.5 - 5.0 mmol/L
Potassium Crit
<2.6 OR >6.2 mmol/L
Ref: Chloride
96 - 109 mmol/L
Ref: Calcium
2.10 - 2.60 mmol/L
Calcium Crit
<1.65 OR >3.25 mmol/L
Ref: Magnesium
0.70 - 1.00 mmol/L
Magnesium Crit
<0.40 OR >1.90 mmol/L
Ref: Phosphorus (phosphate)
0.80 - 1.45 mmol/L
Phosphorus Crit
<0.40 mmol/L
Ref: Anion Gap
4 - 16 mmol/L
Ref: Osmolality (serum)
280 - 300 mmol/kg
Ref: Osmolal gap
<10 mOsm/kg
Ref: Serum total protein
64 - 84 g/L
Ref: Serum albumin
30 - 45 g/L
Ref: 24 hr Urine Total Protein
<0.5 g/volume
Ref: CSF total protein
0.15 - 0.45 g/L
Concentration of dsDNA when A(260) = 1.000
50 µg/mL
Concentration of ssDNA when A(260) = 1.000
33 µg/mL
Concentration of ssRNA when A(260) = 1.000
40 µg/mL
Which formula is used to calculate the reagent amounts required for endpoint PCR ?
C1V1 = C2V2
How is Tm of PCR primers calculated ?
Tm = 4(G+C) + 2(A+T)
What A(260/280) is considered “pure” for DNA ?
~1.8
What A(260/280) ratio is considered “pure” for RNA ?
~2.0
What A(260/230) ratio is considered “pure” for both DNA and RNA ?
Approximately 2.0 - 2.2
Ref: ALP
Female: 30 -130
Male: 30 - 130
Ref: LD
Female: 100 - 225
Male: 100 - 225
Ref: ALT
Female: <40
Male: <60
ALT - alanine transaminase