Red Cell Suspension - Lab Flashcards
Differentiate serum and plasma
serum - without anticoagulant (red top)
plasma - with anticoagulant (EDTA)
purpose of RBC suspension
wash off excess protein and fibrin clots to eliminate false reactions
this is allowed to stand, clot, and centrifuge to extract serum
blood without anticoagulant
what test will be affected if red cell suspension is not properly performed?
Tests involving agglutination reaction
materials/reagent used in rbc suspension
- blood sample
- buffered normal saline solution
- test tubes
- calibrated plastic pipet (0.5 - 3ml)
- serologic centrifuge
Quality control
- buffered NSS is not contaminated
- vacutainer / tubes must not be expired
specimen needed
EDTA anticoagulated blood and serum
transfer ____ in a ____ test tube
transfer 2-3 drops of whole blood in a 10x7 mm tube
wash red cell three times with buffered normal saline. How?
- Fill the tube 3/4 with NSS
- centrifuge for 3 mins
- decant and make sure that the precipitate will remain
after 3rd wash, what’s the next step?
Transfer 1 drop of blood and 19 drops of buffered normal saline in a tube
After transferring the 1:20 ratio blood to nss, what’s the next step?
Put a parafilm and invert gently. Compare your prepared red cell and commercially prepared reagent red cell
what is the normal result?
Bright red
Normal saline should be ___
0.85 - 0.90 % isotonic
what happens is you use a hypertonic nss to your sample?
Cells will shrink (crenated rbc)
what happens if you use hypotonic solution of NSS?
Cells will swell
