crossmatching Flashcards
minor C
what is the principle?
this detects the presence of antibodies in the donor’s serum that will damage or destroy the patient’s RBCs.
minor C
materials needed
- 5 mL test tube
- NSS
- Water bath
- AHG reagent
- centrifuge
- microscope
- donor’s serum
- patient’s RBCs
a clotted or anticoagulated blood drawn within — of testing may be used.
72 hrs
— must be used to assure the presence of adequate complement and calcium. if — used, complement-dependent antibodies may not be detected
FRESH SERUM: to assure presence of complement and calcium.
PLASMA: complement-dependent antibodies may not be detected.
minor C - immediate spin/saline phase
- place — of donor’s serum in a 5 ml test tube.
- add — of 3-5% patient’s red cells.
- mix and centrifuge at — for — then read for the presence of agglutination or hemolysis.
2 drops donor serum
1 drop patient’s RBCs
3400 rpm for 15 secs
minor C - protein phase/thermal phase
- add — of —
- mix and incubate at — for —
- centrifuge for — at — then observe for agglutination or hemolysis.
2 drops of 22% bovine albumin
incubate at 37C for 15-30 mins
3400 rpm for 15 secs
minor C - AHG phase
- wash with — three to four times
- add — of AHG reagent
- mix and centri for — at —
- read for hemolysis or agglutination with or without microscope
- add — if negative
- wash with NSS
- add 2 drops of AHG
- mix and centri for 15 secs at 3400 rpm
- add 1 drop check cells if nega
minor C
this indicates that it is incompatible
agglutination or hemolysis
minor C
this indicates that it is compatible
no agglutination/hemolysis
minor crossmatching is not commonly done these days due to the –
performance of antibody screening and antibody identification
major C
what is the principle?
detect antibodies in patient’s serum that may possibly damage or destroy the donor’s RBCs
major C
materials
- test tube
- NSS
- water bath
- AHG reagent
- centrifuge
- microscope
- patient’s serum
- donor’s RBCs
major C
- 2 drops patient’s serum
- 1 drop 2-5% donor’s red cells
- mix and centri for 15 secs at 3400 rpm
- read for agglutination/hemolysis
identify what phase
immediate spin/saline phase
major C
enumerate the procedure for incubation at 37c or protein phase
- 2 drops of 22% bovine albumin or LISS or PEG
- mix and incubate at 37C for 15-30 mins
- centrifuge for 15 secs then observe
major C
- wash — with NSS
- add — of AHG reagent
- centri for — at —
- read and observe
- test all negative with —
identify what phase
ANTIGLOBULIN PHASE
1. three to four times
2. 2 drops
3. 15 secs @ 3400 rpm
4. check cells
