demonstration of immunohematologic reaction

Question 1

clumping of cells due to antigen-antibody reaction

Answer 1

agglutination

Question 2

since agglutination involves red cells, this should be properly termed as __

Answer 2

hemagglutination

Question 3

destruction of red cells with subsequent release of hemoglobin to the surrounding medium

Answer 3

hemolysis

Question 4

materials needed

Answer 4

• nescofilm
• gum label
• microscope
• test tube
• test tube racks
• pasteur pipet
• glass slides
• applicator sticks
• gloves
• centrifuge

Question 5

reagent/s sample needed

Answer 5

• normal saline solution in a wash bottle
• red cell suspension (ABO rh positive)
• typing sera (anti D) undiluted and diluted 1:10 (1 ml anti-D + 9 ml NSS)

Question 6

objective used for observing the result

Answer 6

LPO (10x)

Question 7

after observing the result using a microscope, what is the next step?

Answer 7

interpret the result within 2 mins as positive or negative.

Question 8

use of anti-D undiluted and diluted, identify the method

Answer 8

tube method

Question 9

for the tube method, how many drops of anti-D is needed for the tube?

Answer 9

2 drops for undiluted and diluted tube

Question 10

how many drop/s of red cell suspension of an Rh + in each test tube?

Answer 10

1 drop

Question 11

centrifuge time and rpm for tube method

Answer 11

15 seconds at 3,400 rpm

Question 12

for demonstration of hemolysis or hemolytic reaction, what are the needed samples/reagent for this method

Answer 12

• 2 drops red cell suspension Rh positive
• 2 drops distilled water in P tube
• 2 drops of NSS in N tube

Question 13

centrifuge time and rpm for the demonstration of hemolysis

Answer 13

15 secs at 3,400 rpm

Question 14

which method you will dislodge the cell button after centrifuge?

Answer 14

tube method

Question 15

for interpretation, no hemolysis indicates?

Answer 15

intact cell button with clear supernatant

Question 16

for interpretation, partial hemolysis should show __

Answer 16

presence of cell button with pink supernatant