Red Cell Suspension Flashcards
provide the appropriate serum-to-cell ratio to allow for grading and interpretation of test results.
Red cell suspensions
RCS
• An______ source for the antigen-antibody reactions
antigen
• A common reagent used for many serologic procedures
RCS
RCS
• An important reagent used for…
confirmation, screening, and identification of antibodies
IMPORTANCE
RED CELL WASHING
- To remove substances that may interfere with serologic tests
substances that may interfere with serologic tests
• Residual plasma and cellular debris
• Wharton’s jelly, cold-reacting autoimmune antibodies, and increased (1) levels of immunoglobulins
• Hemolyzed red blood cells
• Fibrinogen
T or F
One drop of the red cell suspension is going to be equivalent to the amount of antibody that could be present in one drop of the reagent.
True
RBC Washing In Transfusion Medicine
→ There could be instances that a physician is going to request the washing of red cells prior to its transfusion to their patients. This type of blood component is commonly indicated for patients who have:
• Severe allergic reactions
• IgA deficiency
• Febrile transfusion reactions
• Neonates with renal failure
METHODS
RED CELL WASHING
• Manual Washing
• Automated Cell Processor
METHODS
• Can only be performed if you are going to wash a small amount of Red cells
2 mL or up to 5 mL of Red Cells/blood sample contained in a particular blood tube
Manual Washing
METHODS
Washed a unit of packed RBC which might be used particularly for transfusion
PURPOSE: Maintain the sterility of red cells in the blood bank
• Automated Cell Processor
• Reagent to be used for washing
NSS (Normal Saline Solution)
Result coming out of the machine
• To be used for transfusion
Washed RBC
Result coming out of the machine
• To be used for transfusion
Washed RBC
SPECIMEN
RED CELL WASHING
• Blood in EDTA tube
RED CELL WASHING
• It has osmotic pressure that is equal to that of the red blood cells
Same salinity as the blood plasma
NORMAL SALINE SOLUTION
NSS
Has_____ salinity - has the osmotic pressure equal to that of the red blood cells
0.9%
If the salinity of a reagent is lower than 0.9%, the solution becomes …
In this solution, the external environment of the RBC has a lesser salinity compared ot the internal environment of the red cell
HYPOTONIC SOLUTION
If the salinity of the reagent is higher than 0.9%, then most likely the solution is going to be a …
• The external environment of the red cell has higher salinity compared to its internal environment
• The external environment has higher salinity, therefore water inside the cell is going to go out - leading to shrinkage of the red cells
HYPERTONIC SOLUTION
RED CELL SUSPENSION
ERRORS
• Too concentrated (_____)
• Too much antigen (____)
• May result in a _____reaction
> 5%
Post-zone
FALSE NEGATIVE
RED CELL SUSPENSION
ERRORS
Too diluted (____)
Less antigen (____)
May result in_____ reaction
<2%
Pro-zone
FALSE NEGATIVE
RED CELL SUSPENSION
• FORMULA
% RBCs = PCV/TV x 100
PCV =
TV
amount of RBC
total volume (RBC + NSS)
Procedure
