DONOR PROCESSING: INFECTIOUS DISEASE SCREENING

Question 1

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
• VIRAL HEPATITIS

Hepatitis B virus

Hepatitis C virus

Answer 1

• HBsAg
• Anti-HBc (IgG & IgM)

• Anti-HCV
• NAT

Question 2

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
• HUMAN IMMUNODEFICIENCY VIRUS
Screening tests:

Answer 2

• EIA (Enzyme Immunoassay)
• ChLIA (Chemiluminescent Immunoassay)
• NAT (Nucleic Acid Test)

Question 3

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
• HUMAN IMMUNODEFICIENCY VIRUS

Confirmatory Test:

Answer 3

• Western Blot
• Immunofluorescence assay
• Philippines: rHIVda

Question 4

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
• HUMAN T-CELL
LYMPHOTROPHIC VIRUS
: Steen/n
Screening test

Answer 4

• EIA
• ChLIA

Question 5

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
• HUMAN T-CELL
LYMPHOTROPHIC VIRUS

Confirmatory

Answer 5

• Western blot
• Immunofluorescence assay

Question 6

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING
SYPHILIS
• Screening tests

Answer 6

• Rapid plasma reagin (RPR)
• Venereal Disease Research
Laboratory (VDRL)

Question 7

DONOR PROCESSING: INFECTIOUS DISEASE SCREENING

• ADDITIONAL

Answer 7

• West Nile Virus
• Cytomegalovirus
• Chagas Disease
• Malaria
• Babesiosis
• Prion Disease

Question 8

DONOR PROCESSING
BACTERIAL CONTAMINATION
• Most blood units at risk:
• Most common reactions:

Answer 8

PLATELETS (normal skin flora)

Fever, Shock, DIC (Disseminated Intravascular Coagulation )

Question 9

DONOR PROCESSING
• TRANSFUSION SERVICE TESTING
• The only repeat testing required is:

Answer 9

• ABO on red cell products
• D typing (IS) on D-negative red cell products

Question 10

Plasma products (3) do not require any testing.

Answer 10

(FFP, CRYO, PLTS)

Question 11

• Donor samples must be stored at ____for at least __after transfusion

Answer 11

1-6°C

7 days