Recombinant DNA Flashcards
1
Q
What is recombinant DNA technologies?
A
Transferring genes from one organism into a micro-organism
2
Q
What is a vector?
A
A DNA molecule used to carry foreign genetic information into another cell
3
Q
What is restriction endonuclease?
A
An enzyme that cuts nucleic acid
4
Q
What is ligase?
A
An enzyme that joins DNA strands together at the sugar phosphate backbone
5
Q
Describe RDT
A
-Gene transferred into micro-organism that makes required protein
-Restriction endonuclease used to cut out gene and plasmid
-Genes introduced to increase yield or prevent microbe from surviving in external environment
-Ligase seals the enzyme into the plasmid
-Recombinant yeast cells overcome polypeptides being incorrectly folded
-Regulatory sequences are used in plasmids to control gene expression
