REAGENT PREPARATION Flashcards

1
Q

Solution

A

Mixture of substances

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2
Q

Solute

A

Substance that dissolves

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3
Q

Solvent

A

Substance that dissolves solute

- usually water

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4
Q

Dilute solutions

A

Has small portion of solute in solution

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5
Q

Concentrated solutions

A

Contains large portion of solute in solution

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6
Q

Diluent

A

Agent that dilutes a fluid

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7
Q

Dilution

A

Adding diluent to change a concentration of a solution to a lower concentration

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8
Q

What is the most common solvent in clinical labs?

A

water

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9
Q

What water purity has the best quality (minimum impurities)?

A

Type I

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9
Q

What type of water is used to prepare for chemical testing, preparation of reagents, and controls?

A

Type I

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10
Q

What water purity is “good” quality and used for preparing solutions?

A

Type II

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11
Q

What type of water purity has the lowest quality?

A

Type III

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12
Q

How is type I and type II water?

A

Type III water

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13
Q

How is the purity of water determined?

A

The amount of electrical resistance

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14
Q

How is water purity measured?

A

Mega ohms

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15
Q

What type of water has higher electrical resistance and why?

A

Water that is purer as it has less dissolved particles.

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16
Q

What is the five steps of water purification?

A
  1. Distillation
  2. Deionization
  3. Reverse osmosis
  4. Carbon absorption
  5. Filtration
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17
Q

What grade of chemicals have the highest purity?

A

Analytical and reagent

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18
Q

Commercial grade chemicals have ___ degree of impurities.

A

High

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19
Q

What is the procedure that’s “diluting up to the final volume”?

A

% weight per volume

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20
Q

What procedure is referred to as “diluting to volume”?

A

% volume per volume

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21
Q

What is Avogadro’s number?

A

6.022 x 10^23

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22
Q

How do you find molarity of a solution?

A

It’s then # of mols of solute per litre of solution (mol/L = M)

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23
Q

What is normality?

A

It is the unit of concentration describing acids and bases; number of moles equivalent to hydrogen or hydroxide ion per litre of solution

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24
Q

Caustic reagent is added TO the water. (T/F)

A

True

25
Q

WHMIS is just provincially legislated. (T/F)

A

False — also federally legislated

26
Q

What is the four primary components of WHMIS?

A
  1. Hazard identification and classification
  2. Safety data sheet (SDS)
  3. Labelling
  4. Worker education
27
Q

What are the two hazard groups that classifies hazardous substances?

A

Physical hazards and health hazards

28
Q

Hazard categories are numbered from 1 to 4. Which is the least and which is the most hazardous?

A
1 = most
4 = least
29
Q

How many classes are within the physical hazard group?

A

19 classes

30
Q

How many classes are within the health hazard group?

A

12 classes

31
Q

What are some examples of what’s considered a health hazard effect?

A

Skin irritation, carcinogenicity and toxicity

32
Q

What are examples of effects of physical and chemical properties?

A

Flammability and reactivity

33
Q

What 16 headings are included in the SDS?

A

Identification, hazard identification, composition, first aid measures, fire fighting measures, accidental release measures, handling and storage, exposure control/personal protection, physical and chemical properties, stability and reactivity, toxicological info, *ecological info, *disposal considerations, *transport info, *regulatory info, *other info

34
Q

What is found on supplier labels?

A
  1. Product identifier
  2. Pictogram
  3. Signal word (danger or warning)
  4. Hazard statements (fatal, toxic, harmful)
  5. Precautionary statements
  6. Supplier identifier
35
Q

When is a complete workplace label not required?

*but labeling of the container being use is a must

A
  • product poured into a container for immediate use

- product is in the control of the person who aliquoted it

36
Q

What is needed on a workplace label at SAIT?

A

Concentration of the product (matching SDS name)
PPE instructions + pictogram
SDS available
Who and when was it prepared

37
Q

How do you properly transport compressed gas?

A
  • removing the regulator and putting on the protection cap
  • use of a trolley and safety straps
  • no striking of tanks
38
Q

What is the flammability range?

A

Range of vapour concentration per amount of air which the mixture will burn

39
Q

How is the flammability range expressed?

A

Vapour concentration/O2 concentration

40
Q

If a mixture is “too rich”, why will it not burn?

A

Because the concentration of oxygen is too low, therefore, it won’t ignite

41
Q

What is a flash point?

A

the lowest temperature at which liquid gives off vapour near the surface to form a flammable mixture with the air

42
Q

lower the flash point, the ____ the danger

A

greater

43
Q

what is the flash point for ether?

A

-45c

44
Q

what is the flash point for acetone?

A

-20c

45
Q

what is the flash point of toluene?

A

-4c

46
Q

what is the flash point of alcohol?

A

+12c

47
Q

what is the flash point of xylol?

A

+25c

48
Q

What kind of fire extinguishers do you use for flammable liquids?

A

extinguished with a CO2 or ABC fire extinguisher

49
Q

What are some examples of acids that are corrosive?

A
  • hydrochloric acid (HCl)
  • sulfuric acid (H2SO4)
  • nitric acid (HNO3)
  • glacial acetic acid (CH3COOH)
50
Q

What are some examples of bases?

A
  • sodium hydroxide (NaOH)
  • Potassium hydroxide (KOH)
  • ammonium hydroxide (NH4OH)
  • sodium hypochlorite (NaClO)
51
Q

What’s the difference between LD50 and LC50?

A

LD50 = dose = quantity of the substance expected to cause death

LC50 = concentration = concentration of substance in air that when inhaled over a period of time to cause death of 50% of an animal population

52
Q

What is LD50 measured in? LC50?

A
LD = mg (or g) per kg of animal body weight
LC = units of parts per million or mg/M^3
53
Q

How can toxic substances enter the body?

A
  • inhalation, ingestion and absorption through the skin
54
Q

What does threshold limit value (TLV) and permissible exposure limit (PEL) mean?

A

it indicates the maximum limit of exposure to an air contaminant

55
Q

What are the three ways that TLV/PEL can be expressed?

A
  1. 8-hour TWA (time-weighted average)
  2. STEL (short term exposure limit)
  3. Ceiling limit
56
Q

Risk of handling chemicals _____ to the length and frequency of exposure as well as the concentration of the chemical.

A

proportional

57
Q

What does 8-hour TWA indicate?

A

average concentration over 8 hours and its condition to show that worker are exposed repeatedly to these environments without adverse health effects

58
Q

What does STEL indicate?

A

the max concentration that workers can be exposed to something for up to 15 minutes without adverse health effects

59
Q

What does ceiling limit indicate?

A

concentration of airborne substances that can’t be exceeded

- applied to many chemicals with acute toxic effects

60
Q

how is hydrocyanic acid vapours formed?

A

by mixing solutions that contain cyanide with acids

61
Q

What are the four chemicals commonly used in a lab?

A
  • acetone
  • formaldehyde
  • methanol
  • xylene