REAGENT PREPARATION Flashcards
Solution
Mixture of substances
Solute
Substance that dissolves
Solvent
Substance that dissolves solute
- usually water
Dilute solutions
Has small portion of solute in solution
Concentrated solutions
Contains large portion of solute in solution
Diluent
Agent that dilutes a fluid
Dilution
Adding diluent to change a concentration of a solution to a lower concentration
What is the most common solvent in clinical labs?
water
What water purity has the best quality (minimum impurities)?
Type I
What type of water is used to prepare for chemical testing, preparation of reagents, and controls?
Type I
What water purity is “good” quality and used for preparing solutions?
Type II
What type of water purity has the lowest quality?
Type III
How is type I and type II water?
Type III water
How is the purity of water determined?
The amount of electrical resistance
How is water purity measured?
Mega ohms
What type of water has higher electrical resistance and why?
Water that is purer as it has less dissolved particles.
What is the five steps of water purification?
- Distillation
- Deionization
- Reverse osmosis
- Carbon absorption
- Filtration
What grade of chemicals have the highest purity?
Analytical and reagent
Commercial grade chemicals have ___ degree of impurities.
High
What is the procedure that’s “diluting up to the final volume”?
% weight per volume
What procedure is referred to as “diluting to volume”?
% volume per volume
What is Avogadro’s number?
6.022 x 10^23
How do you find molarity of a solution?
It’s then # of mols of solute per litre of solution (mol/L = M)
What is normality?
It is the unit of concentration describing acids and bases; number of moles equivalent to hydrogen or hydroxide ion per litre of solution