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MEDL 200 - Quiz 2 > Microbiology > Flashcards

Microbiology Flashcards

1
Q

Supports the growth of most organisms

A

nutrient media

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2
Q

when blood or serum is added to enhance growth

A

enriched media

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3
Q

What grows on Mannitol salt agar plates?

A

All Staphylococcus species

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4
Q

What are the four types of culture media?

A
  1. nutrient media
  2. enriched media
  3. selective media
  4. differential media
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5
Q

How is media stored?

A

at 4c and protected from dehydration

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6
Q

What does peptone do in culture media?

A

source of carbon and nitrogen

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7
Q

What does meat infusion do for culture media?

A

enhances the growth of most bacteria

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8
Q

What does sodium chloride do in culture media?

A

makes it isotonic; prevents hemolysis in blood agar

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9
Q

What do buffers do for culture media?

A

maintains a constant pH

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10
Q

What does carbohydrates do for culture media?

A

provides nutrients; to indicate whether bacteria is able to ferment them and produce acid

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11
Q

What does agar do for culture media?

A

gelling agent

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12
Q

Blood agar plates (BAP) uses ____ blood.

A

WHOLE

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13
Q

Chocolate agar plates (CHOC) uses ____ blood.

A

Lysed

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14
Q

Laked agar plates (LBA) uses ____ blood.

A

lysed blood that has been frozen and thawed

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15
Q

What is egg based media used for?

A

to grow tubercle bacilli

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16
Q

How does moist heat kill microorganisms?

A

by coagulation of microbial proteins

17
Q

effective coagulation requires _____ and _____.

A

heat and moisture

18
Q

What is the max temp obtained from boiling and free flowing steam?

A

100c

19
Q

What is the pressure and temperature that lab autoclave operate at?

A

15 pounds and temp of 121c

20
Q

How long does it take to kill microorganisms and their spores at in a lab autoclave?

A

15 minutes

21
Q

What are the two things that condensation accomplishes?

A
  1. penetration

2. sterilization

22
Q

What is penetration in terms of steam?

A

when steam condenses into a smaller volume which results more steam to be drawn into the items being sterilized

23
Q

What is sterilization?

A

heat and moisture from steaming coagulates microbial proteins

MEDL 200 - Quiz 2 (5 decks)

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