Microbiology Flashcards
Supports the growth of most organisms
nutrient media
when blood or serum is added to enhance growth
enriched media
What grows on Mannitol salt agar plates?
All Staphylococcus species
What are the four types of culture media?
- nutrient media
- enriched media
- selective media
- differential media
How is media stored?
at 4c and protected from dehydration
What does peptone do in culture media?
source of carbon and nitrogen
What does meat infusion do for culture media?
enhances the growth of most bacteria
What does sodium chloride do in culture media?
makes it isotonic; prevents hemolysis in blood agar
What do buffers do for culture media?
maintains a constant pH
What does carbohydrates do for culture media?
provides nutrients; to indicate whether bacteria is able to ferment them and produce acid
What does agar do for culture media?
gelling agent
Blood agar plates (BAP) uses ____ blood.
WHOLE
Chocolate agar plates (CHOC) uses ____ blood.
Lysed
Laked agar plates (LBA) uses ____ blood.
lysed blood that has been frozen and thawed
What is egg based media used for?
to grow tubercle bacilli
How does moist heat kill microorganisms?
by coagulation of microbial proteins
effective coagulation requires _____ and _____.
heat and moisture
What is the max temp obtained from boiling and free flowing steam?
100c
What is the pressure and temperature that lab autoclave operate at?
15 pounds and temp of 121c
How long does it take to kill microorganisms and their spores at in a lab autoclave?
15 minutes
What are the two things that condensation accomplishes?
- penetration
2. sterilization
What is penetration in terms of steam?
when steam condenses into a smaller volume which results more steam to be drawn into the items being sterilized
What is sterilization?
heat and moisture from steaming coagulates microbial proteins
