REACTIONS OF CARBOHYDRATES (Activity 2b) Flashcards
Formation of yellow crystal, differentiation of reducing sugars
Osazone test
Formation of brick red precipitate after boiling, presence of reducing sugar and non reducing sugars
Fehling’s test
Formation of brick red precipitate, (blue-green-yellow-orange-red), test for simple carbohydrates and presence of reducing sugars
Benedict’s test
Formation of brick red precipitate after 2min of boiling, test for, reducing monosaccharides
Barfoed’s test
Why do glucose and fructose form the same osazone crystals
Configuration of C1 and C2 atoms that vanishes after hydrolysis, hence they form the same crystal
Difference between benedic’ts and fehling’s
sa alkaline condition ang benedict tapos acidic condition ang barfoed
para madetect ang reducing monosaccharide tapos ang barfoed para i differentiate ang mono vs di
Reduction tests, contains cupric ion
Fehling’s, Benedict’s and Barfoed’s
phenyl hydrazine hydrochloride, sodium acetate, acetic acid
Osazone test
Difference between benedict and barfoed
benedicts - reducing sugar
barfoeds - mono or di
cupric acetate, acetic acid
barfoed’s
anhydrous sodium carbonate, sodium citrate, copper (ii) sulfate
benedict’s
copper ii sulfate (a), sodium potassium tartrate (b)
fehling’s
sodium acetate, phenylhydrazinehcl
ozaone
bundle of hay crystals
glucose, fructose
scattered crystals
xylose