COLOR REACTIONS OF PROTEINS (Activity 6)

Question 1

polymer where its repeating unit are amino acids linked together
by peptide linkage.

Answer 1

protein

Question 2

What are the classification of Protein according to their nutritional value?

Answer 2

Complete protein, Incomplete Protein, or Partially complete protein

Question 3

What is the positive result of lead acetate

Answer 3

black precipitate

Question 4

Test for protein

Answer 4

Biuret test

Question 5

Used for detection of amino acids containing guanidium groups (ex arginine), red solution

Answer 5

Sakaguchi test

Question 6

Color produced by proline in ninhydrin test

Answer 6

yellow

Question 7

Test used to show the extent of hydrolysis of proteins

Answer 7

Biuret test

Question 8

Purple solution, presence of proteins, peptide bonds

Answer 8

biuret

Question 9

deep purple solution, presence of protein, ammonia, primary or secondary amines or amino acid

Answer 9

ninhydrin

Question 10

dark yellow or orange solution, presence of amino acid containing aromatic nucleus like tyrosine, tryptophan, and phenylalanine

Answer 10

xanthoproteic

Question 11

brick red solution, presence of tyrosine, soluble proteins

Answer 11

millons

Question 12

purple ring at the interface of two layers, specific for tryptophan indole group

Answer 12

hopkins-cole

Question 13

detection of cystine cystein s-s group s-h group

Answer 13

lead acetate

Question 14

colorless solution, presence of alkanes or phenols

Answer 14

bromine water

Question 15

cherry red solution, presence of tyrosine and histidine

Answer 15

pauly

Question 16

if the sample is unsaturated Potassium permanganate colorize

Answer 16

brown precipitate

Question 17

if saturated color Potassium permanganate colorize

Answer 17

violet or pink

Question 18

Chemical group responsible for the coloration in HOPKIN’S COLE TEST and BROMINE WATER TEST

Answer 18

Indole ring

Question 19

Chemical group responsible for the coloration in LEAD ACETATE TEST

Answer 19

s-s and s-h group, sulfur-containing amino acids

Question 20

Chemical group responsible for the coloration in XANTHOPROTEIC TEST

Answer 20

aromatic amino acid/ aromatic nucleus

Question 21

Chemical group responsible for the coloration in NINHYDRIN TEST

Answer 21

alpha amino acid

Question 22

Chemical group responsible for the coloration in BIURET TEST

Answer 22

two peptide linkage

Question 23

Chemical group responsible for the coloration in MILLONS TEST

Answer 23

phenol group

Question 24

Chemical group responsible for the coloration in PAULY TEST

Answer 24

aromatic amino acid/ aromatic nucleus

Question 25

Chemical group responsible for the coloration in SAKAGUCHI TEST

Answer 25

amino acid containing guanidine group