COLOR REACTIONS OF PROTEINS (Activity 6) Flashcards
polymer where its repeating unit are amino acids linked together
by peptide linkage.
protein
What are the classification of Protein according to their nutritional value?
Complete protein, Incomplete Protein, or Partially complete protein
What is the positive result of lead acetate
black precipitate
Test for protein
Biuret test
Used for detection of amino acids containing guanidium groups (ex arginine), red solution
Sakaguchi test
Color produced by proline in ninhydrin test
yellow
Test used to show the extent of hydrolysis of proteins
Biuret test
Purple solution, presence of proteins, peptide bonds
biuret
deep purple solution, presence of protein, ammonia, primary or secondary amines or amino acid
ninhydrin
dark yellow or orange solution, presence of amino acid containing aromatic nucleus like tyrosine, tryptophan, and phenylalanine
xanthoproteic
brick red solution, presence of tyrosine, soluble proteins
millons
purple ring at the interface of two layers, specific for tryptophan indole group
hopkins-cole
detection of cystine cystein s-s group s-h group
lead acetate
colorless solution, presence of alkanes or phenols
bromine water
cherry red solution, presence of tyrosine and histidine
pauly
if the sample is unsaturated Potassium permanganate colorize
brown precipitate
if saturated color Potassium permanganate colorize
violet or pink
Chemical group responsible for the coloration in HOPKIN’S COLE TEST and BROMINE WATER TEST
Indole ring
Chemical group responsible for the coloration in LEAD ACETATE TEST
s-s and s-h group, sulfur-containing amino acids
Chemical group responsible for the coloration in XANTHOPROTEIC TEST
aromatic amino acid/ aromatic nucleus
Chemical group responsible for the coloration in NINHYDRIN TEST
alpha amino acid
Chemical group responsible for the coloration in BIURET TEST
two peptide linkage
Chemical group responsible for the coloration in MILLONS TEST
phenol group
Chemical group responsible for the coloration in PAULY TEST
aromatic amino acid/ aromatic nucleus
Chemical group responsible for the coloration in SAKAGUCHI TEST
amino acid containing guanidine group
