BIOCHEMICAL PROCESSES (Activity 1) Flashcards
semi-permeable
membrane
cell membrane
Cell membrane separates the ____ from the ____.
cytoplasm, fluid of the outside cell
Water moves from a region of higher concentration to lower concentration or from a region of more dilute solution to a more concentrated solution
osmosis
The movement of some but not all of the dissolved substance
dialysis
the interpenetration of molecules between two substances. substances is continuously occurring in the different cells of the body.
Diffusion
what is the ano in the dialysis
cellophane
solution used in biuret and silver nitrate test
albumin-nacl solution
reagents in biuret test
naoh (sodium hydroxide), cuso4 (copper sulfate)
reagents in silver nitrate test
agno3 (silver nitrate)
what is found in the silver nitrate test
color of the precipitate
rank the rate of diffusion:
a. copper sulfate
b. prussian blue
c. methyl orange
a. 1
b. 3
c. 2
net movement of water molecules through the cell membrane from
an area of higher to an area of lower water potential.
Osmosis
physical process that refers to the net movement of molecules from a
region of high concentration to one of lower concentration.
Diffusion
lower free water molecule concentration than cytoplasm, cell shrinks
hypertonic
higher free water molecule concentration than cytoplasm, cell swells
hypotonic
same free water molecule concentration as cytoplasm, cell stays same size
isotonic
used to determine the presence of a peptide bond in a
substance.
Biuret test
based on the biuret reaction in which a peptide structure containing at least
two peptide links produces a ___ color when treated with alkaline copper sulfate.
violet color
In
presence of an alkaline solution, ___-colored copperII ion can form a complex with the
peptide bonds since the peptide has unshared electron pairs innitrogenandoxygen of
water.
blue
negative result of biuret test
color blue, absence of proteins
positive result of biuret test
color deep purple, presence of proteins
positive result of silver nitrate test
white precipitate
to ml of the albumin-NaCl solution, add a few drops of
AgNO 3 . Observe and note color of precipitate. Repeat using water instead of the
albumin-NaCl.
Silver nitrate test
To 1 ml of the albumin-NaCl solution, add 10 drops of 5N NaOH and
3 drops of 5% CuSO 4. Mix and shake gently. Repeat using water instead of the
albumin-NaCl.
Biuret test
Albumin-Nacl solution result in Biuret and Silver nitrate test
deep purple precipitate and white precipitate
