BIOCHEMICAL PROCESSES (Activity 1)

Question 1

semi-permeable

membrane

Answer 1

cell membrane

Question 2

Cell membrane separates the ____ from the ____.

Answer 2

cytoplasm, fluid of the outside cell

Question 3

Water moves from a region of higher concentration to lower concentration or from a region of more dilute solution to a more concentrated solution

Answer 3

osmosis

Question 4

The movement of some but not all of the dissolved substance

Answer 4

dialysis

Question 5

the interpenetration of molecules between two substances. substances is continuously occurring in the different cells of the body.

Answer 5

Diffusion

Question 6

what is the ano in the dialysis

Answer 6

cellophane

Question 7

solution used in biuret and silver nitrate test

Answer 7

albumin-nacl solution

Question 8

reagents in biuret test

Answer 8

naoh (sodium hydroxide), cuso4 (copper sulfate)

Question 9

reagents in silver nitrate test

Answer 9

agno3 (silver nitrate)

Question 10

what is found in the silver nitrate test

Answer 10

color of the precipitate

Question 11

rank the rate of diffusion:

a. copper sulfate
b. prussian blue
c. methyl orange

Answer 11

a. 1
b. 3
c. 2

Question 12

net movement of water molecules through the cell membrane from
an area of higher to an area of lower water potential.

Answer 12

Osmosis

Question 13

physical process that refers to the net movement of molecules from a
region of high concentration to one of lower concentration.

Answer 13

Diffusion

Question 14

lower free water molecule concentration than cytoplasm, cell shrinks

Answer 14

hypertonic

Question 15

higher free water molecule concentration than cytoplasm, cell swells

Answer 15

hypotonic

Question 16

same free water molecule concentration as cytoplasm, cell stays same size

Answer 16

isotonic

Question 17

used to determine the presence of a peptide bond in a

substance.

Answer 17

Biuret test

Question 18

based on the biuret reaction in which a peptide structure containing at least
two peptide links produces a ___ color when treated with alkaline copper sulfate.

Answer 18

violet color

Question 19

In
presence of an alkaline solution, ___-colored copperII ion can form a complex with the
peptide bonds since the peptide has unshared electron pairs innitrogenandoxygen of
water.

Answer 19

blue

Question 20

negative result of biuret test

Answer 20

color blue, absence of proteins

Question 21

positive result of biuret test

Answer 21

color deep purple, presence of proteins

Question 22

positive result of silver nitrate test

Answer 22

white precipitate

Question 23

to ml of the albumin-NaCl solution, add a few drops of
AgNO 3 . Observe and note color of precipitate. Repeat using water instead of the
albumin-NaCl.

Answer 23

Silver nitrate test

Question 24

To 1 ml of the albumin-NaCl solution, add 10 drops of 5N NaOH and
3 drops of 5% CuSO 4. Mix and shake gently. Repeat using water instead of the
albumin-NaCl.

Answer 24

Biuret test

Question 25

Albumin-Nacl solution result in Biuret and Silver nitrate test

Answer 25

deep purple precipitate and white precipitate