RBC COUNTING

Question 1

a collection of the materials we use for rbc counting

Answer 1

hemocytometer

Question 2

a thick slide we used with an H shaped GROOVE

Answer 2

neubauer slide

Question 3

a combination of Neubauer slide and a cover slip

Answer 3

Neubauer chamber

Question 4

the red cell count is the number of red cells in ___ cu mm

Answer 4

1 cu mm

Question 5

the rbc counting is the test used to diagnose

Answer 5

polycythemia vera and anemia

Question 6

the pipette used for rbc counting

Answer 6

rbc pipette

Question 7

describe the bulb of the rbc pipette

Answer 7

larger than the bulb of the wbc pipette and is colored red material inside

Question 8

materials and equipment used for rbc counting

Answer 8

anticoagulated blood
rbc pipette
diluting fluid
tally counter
counting chamber
gauze pad
microscope
test tube

Question 9

what is the purpose of diluting fluid in rbc

Answer 9

to preserve the integrity of the cell

Question 10

what is the purpose of the diluting fluid in wbc

Answer 10

to lyse rbc

Question 11

procedure for rbc counting

Answer 11

1. draw blood up to 0.5 mark using the RBC pipette
2. wipe the outside walls of the pipette with a clean gauze
3. Dip the pipette into the diluting fluid, then aspirate the diluting fluid into the pipette slowly until the mixture reaches the 101 mark
4. Gently rotate the pipette to mix the diluting fluid and blood
5. Mix for 5 minutes
6. Discard the first 3-4 drops of the diluted sample
7. Prepare the counting chamber
8. charge both sides of the hemocytometer with a drop of diluted sample and allow to stand for few minutes
9. while keeping the hemacytometer in a horizontal position, place it on the microscope stage
10. Using HPO, count the red cells in the 5 “R” squares of the central secondary square

Question 12

one large square is made up of nine___ squares

Answer 12

1-mm x 1-mm

Question 13

each of WBC squares is divided further to ___squares

Answer 13

16

Question 14

the center square for RBC is subdivided into __ smaller squares

Answer 14

25

Question 15

the distance between each counting surface and coverslip (depth) is _____ mm

Answer 15

0.10

Question 16

the total volume is __ cu mm

Answer 16

9

Question 17

in anemic patient, we need to draw blood up to ___ mark and the diluent to ___ mark

Answer 17

1 mark ; 101 mark

Question 18

in a patient with polycythemia vera or erythremia, we need to draw blood into what mark and the diluent up to ___

Answer 18

0.3 mark; 101 mark

Question 19

dilution vale for anemic patient

Answer 19

1:100

Question 20

dilution for patient with polycythemia vera or erythremia

Answer 20

1:333

Question 21

reference values

Answer 21

female
4.0 - 5.4 10x^12 /L

male
4.6 - 6.0 10x^12/L

Question 22

according to video low rbc is seen in what conditions

Answer 22

anemia
vitamin b deficiency
internal bleeding
kidney diseases
malnutrition

Question 24

according to the video high rbc count is seen in

Answer 24

smoking
congenital heart disease
dehydration
hypoxia
certain lung disease

Question 25

in platelet count, the diluting solution is

Answer 25

1% ammonium oxalate

1 g of AO in 100 ml of distilled water

Question 26

if the platelet count is extremenly low, use

Answer 26

wbc opipette and follow the formula of wbc count and use the mmonium ocalate as dil. solution

Question 27

the low platelet count ranges from

Answer 27

140, 000 - 450, 000 cu.mm

Question 28

low platelt count is also known as

Answer 28

thrombocytopenia

Question 29

low platelet count is seen in

Answer 29

leukemia and certain other cancers
viral infection
respiratory disorders
sepsis
autoimmune disease
medications and drugs
exposure to toxic substances
alcoholism
cirrhosis
iron and folate deficiency
vit b12 deficiency

Question 30

hihh platelent count is also known as

Answer 30

thrombocytosis

Question 31

thrombocytosis is seen in what conditions

Answer 31

bleeding
cancer
certain infectons
iron deficiency
removal of spleen
cetain anemia
certain inflammatory
surgery