RBC COUNTING Flashcards
a collection of the materials we use for rbc counting
hemocytometer
a thick slide we used with an H shaped GROOVE
neubauer slide
a combination of Neubauer slide and a cover slip
Neubauer chamber
the red cell count is the number of red cells in ___ cu mm
1 cu mm
the rbc counting is the test used to diagnose
polycythemia vera and anemia
the pipette used for rbc counting
rbc pipette
describe the bulb of the rbc pipette
larger than the bulb of the wbc pipette and is colored red material inside
materials and equipment used for rbc counting
anticoagulated blood
rbc pipette
diluting fluid
tally counter
counting chamber
gauze pad
microscope
test tube
what is the purpose of diluting fluid in rbc
to preserve the integrity of the cell
what is the purpose of the diluting fluid in wbc
to lyse rbc
procedure for rbc counting
- draw blood up to 0.5 mark using the RBC pipette
- wipe the outside walls of the pipette with a clean gauze
- Dip the pipette into the diluting fluid, then aspirate the diluting fluid into the pipette slowly until the mixture reaches the 101 mark
- Gently rotate the pipette to mix the diluting fluid and blood
- Mix for 5 minutes
- Discard the first 3-4 drops of the diluted sample
- Prepare the counting chamber
- charge both sides of the hemocytometer with a drop of diluted sample and allow to stand for few minutes
- while keeping the hemacytometer in a horizontal position, place it on the microscope stage
- Using HPO, count the red cells in the 5 “R” squares of the central secondary square
one large square is made up of nine___ squares
1-mm x 1-mm
each of WBC squares is divided further to ___squares
16
the center square for RBC is subdivided into __ smaller squares
25
the distance between each counting surface and coverslip (depth) is _____ mm
0.10
the total volume is __ cu mm
9
in anemic patient, we need to draw blood up to ___ mark and the diluent to ___ mark
1 mark ; 101 mark
in a patient with polycythemia vera or erythremia, we need to draw blood into what mark and the diluent up to ___
0.3 mark; 101 mark
dilution vale for anemic patient
1:100
dilution for patient with polycythemia vera or erythremia
1:333
reference values
female
4.0 - 5.4 10x^12 /L
male
4.6 - 6.0 10x^12/L
according to video low rbc is seen in what conditions
anemia
vitamin b deficiency
internal bleeding
kidney diseases
malnutrition
according to the video high rbc count is seen in
smoking
congenital heart disease
dehydration
hypoxia
certain lung disease
in platelet count, the diluting solution is
1% ammonium oxalate
1 g of AO in 100 ml of distilled water
if the platelet count is extremenly low, use
wbc opipette and follow the formula of wbc count and use the mmonium ocalate as dil. solution
the low platelet count ranges from
140, 000 - 450, 000 cu.mm
low platelt count is also known as
thrombocytopenia
low platelet count is seen in
leukemia and certain other cancers
viral infection
respiratory disorders
sepsis
autoimmune disease
medications and drugs
exposure to toxic substances
alcoholism
cirrhosis
iron and folate deficiency
vit b12 deficiency
hihh platelent count is also known as
thrombocytosis
thrombocytosis is seen in what conditions
bleeding
cancer
certain infectons
iron deficiency
removal of spleen
cetain anemia
certain inflammatory
surgery
