HEMOGLOBIN DETERMINATION

Question 1

is the red iron-bearing protein contained
within the erythrocytes in normal blood

Answer 1

hemoglobin

Question 2

It is the carrier of oxygen from the lungs to
the tissue where it readily releases this
oxygen to the tissues and then returns to the
lungs to combine with more oxygen

Answer 2

hemoglobin

Question 3

The cyanmethemoglobin is said to be the method of
choice for hemoglobin determination because

Answer 3

1. Cyanmethemoglobin is stable in dilutions.
2. Cyanmethemoglobin standard are readily available.
3. All hemoglobin derivatives except sulfhemoglobin
   are measured with their conversion to
   cyanmethemoglobin.

Question 4

what is the principle of the hemoglobin determination/ cyanmethemoglobin

Answer 4

Hemoglobin iron is converted from ferrous to
ferric state to form methemoglobin by the
action of ferricyanide

Methemoglobin then combines with
potassium cyanide to produce the stable
cyanmethemoglobin which is measured
spectrophotometrically

Question 5

is fasting needed for hemoglobin determination?

Answer 5

no

Question 6

materials for cyanmethemoglobin

Answer 6

Anticoagulated blood Test tubes
pipette
Spectrophotometer
Drabkin’s Reagent
cuvette

Question 7

procedure for cyanmethemoglobin

Answer 7

1.Place 5 ml of Drabkin’s
reagent into a test tube

2.Using Sahli pipette,
draw blood to 0.02 cc
mark. Make sure to wipe
the outer wall of the
pipette, and then
dispense the blood to
the test tube

3. Gently draw up
   and down to rinse
   the pipette and
   also to mix the
   resulting colloidal
   suspension
4. Cover and mix
   well by inversion .
   Let it stand for 5
   minutes.
5. Transfer the
   mixture to a cuvette
6. Set the spectroto 100%
   transmittance at the wavelength
   of 540 nm. Using a
   cyanmethemoglobin reagent
   blank
7. Continue reading the
   patient’s sample and record the
   percentage transmittance

Question 8

what is the formula for the

Answer 8

Conc of unknown :
abs of
unknown
_________
abs of std

x value of std

Question 9

what is the value of the concentration of the std/ value of the std

Answer 9

12.5

Question 10

abs of std

Answer 10

0.350

Question 11

what is the wavelength used for the Spectrophotometer

Answer 11

540 nm

Question 12

transmittance percentage of specto

Answer 12

100%