Random Questions

Question 1

What are three cell adhesion molecules? how are they different from each other?

Answer 1

Cadherin: Cell-cell adhesion (extracellular connection), Calcium-dependent.

Integrin

Selectin

Selectins - reach out of cell and select what they want to adhere to.

Cadhesions - provide cell adhesion using Calcium.

Desmosome - ”das my zone” - form network of links between adjacent cells (all together look like a zone).

Gap junction - joining cytoplasms, removing the gap between cells. Desmosomes connect cells without joining them, which leaves a gap.

Question 2

Bradford Protein Assay – what is it for and how does it work?

Answer 2

• A colorimetric method to measure protein concentration. the reagent in the Bradford assay turns blue when binding to a protein.
• Absorption –> concentration of blue –> concentration of protein.

Question 3

What are the two possible formations of a beta sheet? which one is stronger? what is the bond that drives the formation of beta sheet? how is that different from alpha helix?

Answer 3

• Beta sheet is formed because of the hydrogen bond interaction between strains while alpha helix is fromed because of the hydrogen interaction within the strain.
• Antiparrall beta sheet is more stable because the hydrogen bond formation is more optimal.

Question 4

what is the name of the RNA before slicing that exist inside the nucleus?

Answer 4

heterogeneous nuclear RNA