Rabies

Question 1

Virion

Answer 1

Helical symmetry
Enveloped
G proteins

Question 2

Not- endemic

Answer 2

Australia (bat lyssavirus)
New Zealand
Great Britain
Ireland
Hawaii
Japan

Question 3

N, P, M, G, L

Answer 3

N - Nucleoprotein gene
P - Cofactor of Polymerase
M - Inner virion protein used in budding
G - Glycoprotein that forms trimers - spikes
L - RNA - dependent - RNA - Polymerase

Question 4

CNS

Answer 4

Minimal Histological sings
Negri bodies

Question 5

Deaths

Answer 5

55K wordwide
2 in US

Question 6

Canine Rabies

Answer 6

Eliminated in 1970s

Question 7

Wildlife species

Answer 7

Raccoons
Bats
Skunks
Fox

Question 8

Not common in

Answer 8

Rodents
Squirrels
Swine

Question 9

Stability

Answer 9

Cool
Moist
Alkaline pH

Question 10

Cold Chain

Answer 10

4C
50% glycerol in saline

Question 11

Classification

Answer 11

Rhabdo - Lyssavirus

Question 12

Genotypes

Answer 12

Based on N protein

7 Genotypes
-Classical
-Lagos - No human disease
-Mokola
-Duvenhage
-European bat lyssavirus 1 and 2
-Australina bat lyssavirus

4 Proposed
-Aravan
-Irkut
-Khujand
-West Caucasian Bat

Question 13

Serogroup

Answer 13

Only ONE major Serogroup

Question 14

Variants

Answer 14

Distinguished by G and G-L, N
Panel of monoclonal AB to N

Question 15

Biotypes

Answer 15

Variants associated with Specific species
Highly adapted to species

Question 16

Animal Variants

Answer 16

3 skunk
3 fox
1 coyote
1 racoon
2 bat
1 mongoose (PR)
Zero Dog

Question 17

Cycle

Answer 17

1. Enter myocytes via nAchR
2. Rep in myocytes -> Motor neuron NCAM
3. Axonal Movement to CNS
4. Limbic System - Furious - > Neocortex - Dumb
5. Centrifugal spread to peripheral nerves

Question 18

Centrifugal spread from CNS

Answer 18

Salivary glands
Cornea
Internal organs
Nerve bundles in sensory hairs
Body Fluids
Death by respiratory arrest/paralysis

Question 19

Saliva

Answer 19

In saliva 10 days before overt signs (10 day quarantine)
Furious form may or may not be manifested

Question 20

Incubation

Answer 20

2 - 3 weeks up to 6 months or years

Question 21

Death

Answer 21

2 - 14 days after onset

Question 22

Additional signs

Answer 22

Can’t swallow
Pytalism (salivation)
Protrusion nictitating membrane
Hyperesthesia
Self Mutilation
Pica
Vocalization
Racoon - incoordination

Question 23

Histologically

Answer 23

Eosinophilc cytoplasmic inclusions
Negri bodies
Ammons horn

Question 24

Diagnosis

Answer 24

Presumptive - abnormal behavior
Definitive - FAT
Mouse inoculation MIT
VI using BHK
PCR - Negative not meaningful

Question 25

Control

Answer 25

Animal control
Vaccination of wildlife
Vaccination of domestic

Question 26

Exposure

Answer 26

10 day quarantine
Kill and submit to lab
Treatment if RV not ruled out