Quiz 8

Question 1

The DNA profiles used as evidence in a murder trial look something like supermarket bar codes. The pattern of bars in a DNA profile shows.

Answer 1

The presence of various sized fragments of DNA

Question 2

When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work because.

Answer 2

the fragments will bond to other fragments with complementary ends.

Question 3

In PCR, Taq polymerase is used opposed to E.Coli DNA polymerase because

Answer 3

Only Taq polymerase can survive the high temperatures used in PCR

Question 4

The following is NOT an application where one would use PCR.

Answer 4

Distinguishing between consubstantiation and sacramental union.

Question 5

In order to perform the polymerase chain reaction, one needs to use short single-stranded DNA primers. Why is the nucleotide sequence of these DNA primers so important?

Answer 5

In PCR, the goal is to make countless copies of a specific region of the DNA. The primers are complimentary to the beginning and end of these regions, and allow the Taq polymerase to copy the these specific regions.