Quiz 8

Question 1

The process of deleting or rendering a gene functionally inactive is called __________.

Knock out
Knock in
Knock down
Knock through

Answer 1

Knock out

Question 2

What is an advantage that ZFNs have over TALENs?

ZFNs produce less off-target mutations than TALENs
ZFNs are easier to design than TALENs
ZFNs are 3 times smaller than TALENs, allowing for easier delivery into the host cell
ZFNs have a higher binding specificity to the target sequence than TALENs

Answer 2

ZFNs are 3 times smaller than TALENs, allowing for easier delivery into the host cell

Question 3

Which genome editing technique is specifically for plants?

CRISPR-Cas9
Agrobacterium-mediated gene transfer
Red recombination
Cre-lox recombination

Answer 3

Agrobacterium-mediated gene transfer

Question 4

Which of the following best describes the orientation the loxP sites must be in to result in the deletion of a gene?

loxP sites must be on different DNA strands
loxP sites must be within the gene
loxP sites must be facing opposite directions
loxP sites must be facing the same direction

Answer 4

loxP sites must be facing the same direction

Question 5

Which of the following is not a component of CRISPR-Cas9?

tracrRNA
sgRNA
gam
PAM

Answer 5

gam

Question 6

Which of the following is not a characteristic of cancer cells?

Loss of cell cycle control
Can induce local blood vessel formation
Sensitive to antigrowth factor
Tissue invasion and metastasis

Answer 6

Sensitive to antigrowth factor

Question 7

What is a proto-oncogene?

Genes that code for proteins that help regulate cell growth and differentiation
Genes that code for cancer-inducing proteins
Genes that prevent the expression of DNA repair genes
Genes that promote the duplication of other genes

Answer 7

Genes that code for proteins that help regulate cell growth and differentiation

Question 8

Which gene has not been found to be involved in the development of cancer?

Ras
BRCA1
p53
CFTR

Answer 8

CFTR

Question 9

Which genes play a role in cancer?

DNA repair genes
Growth promoting genes
Growth inhibiting genes
All of the above

Answer 9

All of the above

Question 10

Which of the following is not an approach used by gene therapy?

Using modified viruses to target cancer cells
Changing gene behavior without altering the DNA
Inserting new genes directly into body tissues
Correcting genes outside the body and then putting them back in

Answer 10

Changing gene behavior without altering the DNA