Quiz 6 Flashcards
What is not a function or feature of restriction enzymes
Random alteration of DNA sequences
Recognition of specific sequences of DNA
ecoRI as a common lab enzyme
Double-stranded cuts in DNA at target sequences
Random alteration of DNA sequences
What are the disadvantages of using a single restriction enzyme for plasmid vector digestion and ligation in cloning?
Only one strand of the DNA is cut, leading to incomplete digestion
The gene can be inserted in both directions
Self-ligation of the digested vector
b and c are correct
b and c are correct
What is a key feature of the T5 Exonuclease enzyme in the Gibson Assembly process?
It seals the nicks in the DNA backbone.
It creates single-stranded DNA by removing nucleotides from the 5’ end.
It joins DNA molecules with the addition of extra sequences.
It synthesizes DNA in the non-overlapping “gaps.”
It creates single-stranded DNA by removing nucleotides from the 5’ end.
Which component is unique to the Gibson Assembly technique compared to the Golden Gate Assembly?
5’ exonuclease
DNA insert(s)
Destination vector
T4 DNA ligase
5’ exonuclease
What is a key consideration when using the Golden Gate Assembly method for cloning?
The type IIS restriction site must not be present within the DNA fragments being assembled.
Multiple enzymes are necessary to generate different sticky ends.
The method is 100% sequence-independent, allowing any fragment to be assembled.
It requires a heat-stable type IIS restriction enzyme
The type IIS restriction site must not be present within the DNA fragments being assembled.
What are products that can be produced using synthetic biology?
Aspartame
All of the above
L-glutamate
L-lysine
All of the above
What distinguishes catabolism from anabolism in cellular metabolic pathways?
Catabolism breaks down complex molecules into simple molecules releasing energy, whereas anabolism uses energy to synthesize complex molecules from simple molecules.
Both catabolism and anabolism release energy by breaking down complex molecules into simple molecules.
Anabolism breaks down complex molecules into simple molecules releasing energy, whereas catabolism uses energy to synthesize complex molecules from simple molecules.
Both anabolism and catabolism use energy to build complex molecules from simple molecules.
catabolism breaks down complex molecules into simple molecules releasing energy, whereas anabolism uses energy to synthesize complex molecules from simple molecules.
What distinguishes the production volumes of antibiotics like penicillin compared to those like vancomycin?
Vancomycin has a broader range of applications, so it is produced in larger volumes than penicillin.
Penicillin is produced in very large volumes due to its wide range of applications, whereas vancomycin is produced only in relatively small amounts.
Penicillin is produced only in small amounts due to its limited applications, whereas vancomycin is produced in large volumes.
Penicillin and vancomycin are both produced in very large volumes due to their effectiveness against streptomyces.
penicillin is produced in very large volumes due to its wide range of applications, whereas vancomycin is produced only in relatively small amounts.
Which of the following is NOT a layer of regulation in the L-tryptophan biosynthetic pathway in E. coli?
Repression by the TrpR protein.
Overexpression of the trpEDCBA operon.
Attenuation of the trpEDCBA operon.
Feedback inhibition of DAHP synthases.
overexpression of the trpEDCBA operon.
For the production of synthetic industrial enzymes, microorganisms must be
Derived only from wild-type organisms.
Nontoxic, nonpathogenic, and non-antibiotic-producing
Exclusively from the bacterial kingdom.
Able to produce antibiotics.
nontoxic, nonpathogenic, and non-antibiotic-producing
