Quiz 7

Question 1

What is the goal of synthetic biology?

To understand the historical evolution of different species
To reduce the complexity of biological organisms to simpler forms
To replicate natural biological systems exactly as they are found in nature
To extend or modify the behavior of natural organisms and engineer them to perform new tasks

Answer 1

To extend or modify the behavior of natural organisms and engineer them to perform new tasks

Question 2

What is not a part of the logic gates?

OR
all are correct
NOT
AND

Answer 2

all are correct

Question 3

What specifically is being designed and constructed in the field of new biological engineering?

Only a, b, c are correct
Systems
Machines
Parts
All are correct
Devices

Answer 3

Only a, b, c are correct

Question 4

What is not true of Repressilator?

The Repressilator’s design does not allow for the genes to express proteins that repress the subsequent gene in the loop
A possible application is intermittent administration of a therapeutic protein
It is a genetic regulatory network that includes a feedback loop with at least three genes
Each gene in the Repressilator expresses a protein that represses the next gene in the loop

Answer 4

The Repressilator’s design does not allow for the genes to express proteins that repress the subsequent gene in the loop

Question 5

How can the transition from the high Cl state to another state be induced in the synthetic toggle switch?

By introducing additional LacR molecules or degrading CI
By removing all LacR molecules from the system
By increasing the temperature of the environment
By adding more substrates for the CI protein

Answer 5

By introducing additional LacR molecules or degrading CI

Question 6

Why is protein engineering performed?

Better recombinant expression
a and b are correct
Improve solubility
a, b, and c are correct
increase reaction rates

Answer 6

a, b, and c are correct

Question 7

What can cause proteins to denature?

a and c are correct
Decrease in pH
Increase in pH
Excessive heat
a, b , and c are correct

Answer 7

a, b , and c are correct

Question 8

Which of the following is not true regarding the importance and creation of disulfide bonds?

Site-directed mutagenesis can be done to add two cysteine residues into a protein
Generally, the longer the loop between two cysteines, the more stable the protein
They increase the thermal and solvent stability
Disulfide bonds make a protein more resistant to changes in pH

Answer 8

Disulfide bonds make a protein more resistant to changes in pH

Question 9

What can be added to proteins to facilitate easier purification?

Salt bridges
None of the above
Affinity tags
Disulfide bonds

Answer 9

Affinity tags

Question 10

The place where an enzyme binds to the substrate is called an __________.

Specific site
Activation site
Active site
Attachment site

Answer 10

Active site