Quiz 5

Question 1

What component(s) are used in PCR?

a & b are correct
dNTPs
primers
RNA polymerase

Answer 1

a & b are correct

Question 1

What is the purpose of a selectable marker in a vector?

It possesses unique restriction sites that can be used to insert the gene of interest
It contains the ribosome binding sequence that allows for translation initiation
It reduces unwanted transcription and increases plasmid and mRNA stability
It helps scientists to determine which cells contain the plasmid

Answer 1

it helps scientists to determine which cells contain the plasmid

Question 2

Which of the following is not a step used in Genetic Recombinant Technology?

The gene of interest is inserted into the plasmid through ligation
PCR is used to amplify selectable markers
The presence of the recombinant plasmid is verified in the host cells
The gene of interest and plasmid are cut using restriction enzymes

Answer 2

PCR is used to amplify selectable markers

Question 3

Which of the following is not an essential component of a plasmid?

Origin of replication
Gene of interest
Promoter and terminator
Protease cleavage site

Answer 3

Protease cleavage site

Question 4

Which of the following is not correct regarding conditions to consider when designing a primer?

Avoid repetitive sequences
GC content should be about 40% – 60%
The melting temperature should be 50°C – 55°C
Primers should be 22-26 bases in length

Answer 4

The melting temperature should be 50°C – 55°C

Question 5

Which of the following are advantages of the Baculovirus Expression System?

Since the polyhedron gene promoter is strong, it can produce high levels of recombinant protein
It is capable of post-translational modifications
a and b are true
Baculovirus re-infection of insect cells is not needed to continue protein synthesis

Answer 5

a and b are true

Question 6

What are parameters that should be considered when producing recombinant proteins?

Appropriate screening and scaling up methods
Time spent expressing the protein
Selection of a suitable expression vector
All of the above

Answer 6

All of the above

Question 7

What is the purpose of fusion tags?

Tag proteins for degradation
Purification and detection of recombinant proteins
Tells the host cell to secrete the recombinant protein
Promotes the post-translational modification of recombinant proteins

Answer 7

Purification and detection of recombinant proteins

Question 8

What is a disadvantage of the E. coli expression system?

Slow doubling time
Yields of recombinant proteins are low
Expensive
Degrade the recombinant protein

Answer 8

Degrade the recombinant protein

Question 9

Which of the following does not accurately describe plasmid segregation?

Compatible plasmids can coexist in the same cell by occupying different subcellular locations
When plasmids are incompatible, 1 plasmid will be degraded
When 2 plasmids have the same origin of replication, the replication of 1 plasmid is inhibited due to RNA antisense mechanism
Plasmid segregation can occur if multiple plasmids carry the same antibiotic resistance gene

Answer 9

When plasmids are incompatible, 1 plasmid will be degraded