Quiz 7

Question 1

Found in bacteria to protect against viral infection

they do this by cutting the large viral DNA into smaller, more manageable and harmless fragments

Answer 1

Restriction enzyme

Question 2

They recognize and cut DNA at specific base pair sequences

Answer 2

restriction enzymes

Question 3

Each enzyme generates a unique set of ______ ____ in the DNA strand where they cut, regardless of the DNA source

Answer 3

sticky ends

Question 4

EcoR1 locates this _______ sequence to cut between certain base pairs within any DNA molecule wherever it occurs

Answer 4

specific

Question 5

_______ with EcoR1 creates fragments with sticky ends at either end

Answer 5

Digestion

Question 6

DNA from any source with the same _____ ____ can be ligated or spliced together

Answer 6

sticky ends

Question 7

_______/_____ _____ involves ligating a fragment of DNA to a cloning vector which has been digested with the same restriction enzyme

Answer 7

DNA/gene cloning