Lab Quiz 1 Flashcards

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1
Q

What is prohibited in the lab?

A

No food/drink, no cell phone use, no belongings at lab table

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2
Q

When do you turn in worksheets from the lab?

A

At the next lab session

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3
Q

When are quizzes given?

A

The week after the lab

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4
Q

What are quizzes on?

A

The previous weeks lab and the current lab

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5
Q

Are all quizzes counted?

A

Lowest quiz grade is dropped

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6
Q

What questions on quizzes are from previous labs?

A

1-4

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7
Q

What questions are on the current lab?

A

5-6

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8
Q

What is the attendance policy for labs?

A

Attendance is mandatory for all labs

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9
Q

What do you do if you miss a lab?

A

You must make-up the lab in another session during that SAME week

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10
Q

What is the honor policy regarding labs?

A

Be honest and truthful, turn in your own work/answer questions in your own words

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11
Q

What are the general expectations for labs?

A

Be on time and don’t leave early, be prepared for the lab, have your supplies, complete assignments on time, keep lab table neat/clean, keep belongings in cubbies, be respectful

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12
Q

How can you earn + TA points?

A

Help set up lab, help clean up lab, help during lab, go to office hours, give in depth quiz answers, give good answers during the lecture portion of lab

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13
Q

How can you earn - TA points?

A

Not bringing supplies, not being properly dressed, late/leaving early, using cell phones, not paying attention, not cleaning up, being disrespectful, giving no effort, improper disposal of waste

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14
Q

W/ each tube the substance became more and more diluted

A

Serial dilution

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15
Q

The unknown variable

A

Dependent variable

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16
Q

The known variable

A

Independent variable

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17
Q

In lab 1 the dependent variable was

A

The absorption of visible light

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18
Q

The lab 1 the independent variable was

A

The concentration

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19
Q

What are glass/plastics are used for?

A

Storing stock solution, don’t ever use solution directly from glassware move to smaller container

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20
Q

What is án aliquot?

A

A small amount of stock solution

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21
Q

What are graduated cylinders used for?

A

Measuring large volumes of liquid; if accuracy is critical use the smallest cylinder possible

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22
Q

What is an Erlenmeyer flask used for?

A

To mix solutions

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23
Q

What is a beaker used for?

A

Preparing solutions; not used for storing; do not use for precise measurements

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24
Q

What are test tubes used for?

A

Used to prepare reaction mixtures and for short term storage

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25
Q

What is the sharps box?

A

Where broken glass or disposable glassware and pipettes are discarded

26
Q

What is the needle box?

A

Where razor blades/needles are discarded

27
Q

What is a vortex mixer used for?

A

To mix solutions in test tubes

28
Q

What is a micro centrifuge used for?

A

To spin solutions at high speeds that generate up to 15,000 Gs of force

29
Q

What are water baths used for?

A

Filled with distilled water and are used to incubate reaction mixtures at precise temperatures

30
Q

What is a digital balance used for?

A

Used to weigh materials between 0.01 and 400 grams

31
Q

What are pipettes?

A

They are calibrated in millimeters, tenths, or hundredths and are used to measure and transfer small amounts of liquids

32
Q

Micropipettors are used for what?

A

Taking up small amounts of a substance and transferring it

33
Q

What are the 3 kinds of micro-pipettors?

A

P-20, p-200, p-1000

34
Q

Mu l stands for what?

A

Microliter

35
Q

Micro-pipettors measure in what?

A

Microliters

36
Q

P-20 and P-200 use what tips?

A

The smaller yellow tips

37
Q

P-1000 used what kind of tip?

A

The larger blue tips

38
Q

P-20 is used for volumes in what range?

A

1-20 microliters

39
Q

P-200 is used for what range of volumes?

A

20-200 microliters

40
Q

P-1000 is used for what range of volumes?

A

200-1000microliters

41
Q

How do you read the dial on a micropipette?

A

The three boxes go in descending order
P-20: tens, ones, tenths
P-200: hundreds, tens, ones
P-1000: thiusands, hundreds tens

42
Q

Vo Cf
—- = —-
Vf Co

This equation is used for what?

A

To determine how much stock is needed to prepare a solution if a certain volume and concentration

43
Q

What does Vo stand for?

A

Original volume

44
Q

What does Vf stand for?

A

Final volume

45
Q

What does Co stand for?

A

Original concentration

46
Q

What does Cf stand for?

A

Final concentration

47
Q

How much flavin was used in lab 1?

A

720 microliters

48
Q

How much phosphate buffer was used?

A

3 ml

49
Q

How do you change a volume that is in millimeters to microliters?

A

Move the decimal to the right 3 places

50
Q

What does a spectrometer do?

A

Measures only colored substances; measures how much light is absorbed, transmitted, and reflected

51
Q

What is transmittance?

A

The percent of light that passed through a substance

52
Q

What is the transmittance formula?

A

T = Io
—-
I
Where Io is the incident light

53
Q

How are absorbance and concentration related?

A

They are directly proportional

54
Q

What is variation?

A

Differences among species, individuals within each species, and it occurs at all levels of biological organization

55
Q

What is statistical analysis?

A

Applied mathematics used to test a hypothesis

56
Q

What phylum are pill and sow bugs from?

A

Arthropoda

57
Q

What are pill and sow bugs classified as?

A

Crustacea

58
Q

Describe Arthropoda?

A

Hard exoskeleton, jointed legs, she’d in order to grow

59
Q

Lives on land

A

Terrestrial

60
Q

What is the purpose of lab 2?

A

See if pull and sows bugs can be differentiated by their differing reactions to light

Learn how to use excel on a computer

61
Q

How can you tell pill and sow bugs apart?

A

Pill bugs roll into balls when threatened

62
Q

What is the appropriate clothes to wear to lab?

A

Long pants, closed toe shoes, hair in ponytail