Quiz 3 Flashcards by Melissa Friesen

Who typically requires an antibody identification test?

*Transfusion patient
*OB patient
*Donors

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What is the purpose of antibody identification?

To detect unexpected or atypical antibody in the serum or on the cells that may cause in vivo destruction of red blood cells

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What variations do antibody identification tests have?

Variations in temperature and media of reactivity

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What factors influence antibody frequency?

Antigen frequency
Immunogenicity of antigen
Presence of non-human antigen source similar to blood antigen

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What antibody is most frequently found?

Anti-D

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What are the alleles for the Lewis system?

Le(a)

Le(b)

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What are the main alleles in the Kell system?

K, k

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What are the main alleles in the Duffy system?

Fy(a+b-), Fy(a-b+), Fy(a+b+), Fy(a-b-)

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What are the alleles found in the Kidd system?

Jk(a)

Jk(b)

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What are the alleles of the P system?

P(1)
P(2)
p(k)
p(Tja-neg)

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If an antibody reacts well in saline and at room temperature, what class of antibody is it most likely?

IgM

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If an antibody reacts well at 37C in albumin or after antiglobulin test, what class of antibody is it usually?

IgG

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What antibody can cross the placenta?

IgG

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What antibody system reacts in all temperatures and mediums?

Lewis system

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What antibodies deteriorate in storage?

P, Le(a), Fy(a), Kidd

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What antibodies are destroyed by enzymes?

Duffy, MNSs

What antibodies are enhanced by enzymes?

Rh, Kidd, Lewis, I, P

What antibodies show dosage?

MNSs, Kidd, Rh, Duffy

What antibodies are classified as IgM?

Le(a), Le(b)
P
MN
I, i
ABO

What antibodies are classified as IgG?

D, C, E, c, e 
Jk(a), Jk(b)
k, K
S, s
Fy(a), Fy(b)

How do you perform an autoabsorption?

Patient cells + Patient serum allows for antibodies to be absorbed out of the serum. Then use absorbed serum to test for antibodies.

Procedure used for elution in antibody identification?

Removal of antibodies from red cells; then use eluate to identify the antibody

Lewis system

Function
Optimal temperature
Antibody type
Specific media

Affects if you are a secretor or not
25C
IgM
Saline

Kell system

Antibody class?
Possible causes?
Optimal temperature?
Optimal medium?

IgG
Transfusion reactions & HDN
37C
Anti-human globulin

Duffy system 1. Common in patients with... 2. Antibody class? 3. Possible causes? 4. Optimal temperature? 5. Optimal medium?

1. Multiple transfusions 2. IgG 3. Transfusion reactions and HDN 4. 37C 5. Anti-human globulin

The Duffy system has a relationship with what virus?

Plasmodium vivax --> malaria

Kidd system 1. Antibody class? 2. Found in patients with... 3. Possible causes? 4. Optimal temperature? 5. Optimal media?

1. IgG 2. Multiple transfusions 3. Transfusion reactions and HDN 4. 37C 5. AHG

MNSs system | 1. List the antibodies found in this system

* Anti-M * Anti-N * Anti-S * Anti-s

Anti-M 1. Antibody class? 2. Important feature?

1. IgM | 2. Pronounced dosage effect

Anti-S and anti-s 1. Antibody class? 2. Possible causes? 3. Show dosage? 4. Optimal temperature? 5. Optimal medium?

1. IgG 2. Transfusion reactions and HDN 3. Yes 4. 37C 5. AHG

Anti-M and Anti-N 1. Optimal temperature? 2. Optimal medium?

1. 25C | 2. Saline

P system 1. Antibody class? 2. Optimal temperature? 3. Optimal media? 4. Transfusion reactions and HDN?

1. IgM 2. 25C 3. Saline 4. No

I, i system 1. What antigen is found on normal adult red cells? 2. What antigen is found on normal baby red cells?

1. I | 2. i

Anti-I 1. Causes problems with... 2. Antibody class? 3. Optimal temperature? 4. Optimal media? 5. Possible diseases?

1. Reverse type, antibody screen, and cross match 2. IgM 3. 25C 4. Saline 5. Cold autoimmune hemolytic anemia