Quiz 2 Flashcards
Subcutaneous inject
under skin, vaccines
IM
muscle (used for morphine)
IP
peritoneal cavity (used for all other laps, caffeine is unknown)
Alcohol behavioral tests
open field maze
rotorod/beam
warm plate/temp.
Rats low body temp, worse coordination, high anxiety
Anxiety and Anticipation
Open field maze
Concentrations: Scopolamine Caffeine Ethanol Morphine sulfate Diazepam
S- 1 mg/kg C- 20mg/kg E- 1g/kg, diluted by half distilled water MS- 5mg/kg IM injection D-2.5 mg/kg
Why IM?
slow absorption rate, so safe for rats
Why IP?
Gets there quickly, avoids 1st pass metabolism, quick onset time.
Condition placed preference- morphine
vertical and horizontal slits
8 day training
Subcutaneous injection.
Group A was saline, Group B was both alternated 3mg in 0.3 saline morphine S. in one vs. the other
Serotinin, unlike choline
influenced by food
Light-dark box
Measures anxiety
More time in dark = more anxious
Anxiolytics (GABA/Diazepam lab)
Elevated Plus Maze
Measure anxiety
More time in closed = more anxiety
Anxiolytics (Gaba/Diazepam)
Gaba/anxiolytics
= lower anxiety
Open field maze was used…
Stimulants=10 mins
Alcohol=5 mins
Diazepam = 3 mins
Activity- squares entered
anxiety- number of inner/outer squares
Temperature
base of tail, infrared
alcohol, opiates
Stimulant: increase temp
Alcohol: lower temp
opiates: higher
Rotorod/beam
Alcohol and opiates
worse coordination in both
Warm plate
pain
alcohol and opiates
If you have too many depressants, how do you die?
Respiratory failure
Side effect opiates
constipation
Rats used were
Long Evans rats
What experiments were mixed?
Water maze, morphine
What experiments were between?
Caffeine, Alcohol, Diazepham
proper way to hold rat
base of tail, by scruff of neck, over the neck/shoulders holding from back
Once pipettor is set correctly, no need to check again
false
methods:
subjects, apparatus, procedure
Pipetting solution
Bradford
