Quiz 1: Amino Acids and Proteins

Question 1

This structure is an example of:

(a) Amino Acid
(b) Glycine
(c) Hemoglobin
(d) Protein

Answer 1

(a) Amino Acid

Question 2

Which of the following is an amino acid?

(a) Arachidonic acid
(b) Chylomicron
(c) Glycine
(d) Glycerol

Answer 2

(c) Glycine

Question 3

Which of the following acute-phase reactant proteins decreases during inflammation?

(a) Alpha-1-antitrypsin
(b) Fibrinogen
(c) Haptoglobin
(d) Transferrin

Answer 3

(d) Transferrin

Question 4

Which of the following is an acute-phase reactant protein which increases during inflammation?

(a) Alanine
(b) Glycine
(c) Serum Ferritin
(d) Transferrin

Answer 4

(c) Serum Ferritin

Question 5

Which of the following plasma protein is primarily responsible for maintaining in vivo colloidal osmotic pressure?

(a) Albumin
(b) Alpha-2-Macroglobulin
(c) Fibrinogen
(d) Hemoglobin

Answer 5

(a) Albumin

Question 6

Which of the following describes a peptide bond?

(a) An amino group and carboxyl group bonded to an alpha-carbon
(b) A double carbon bond between the alpha-carbon and two amino acids
(c) An amino group bonded to a carboxyl group of another amino acid
(d) Two carboxyl groups bonded to the alpha-carbon of an amino acid

Answer 6

(c) An amino group bonded to a carboxyl group of another amino acid

Question 7

Which of the following conditions would indicate a plasma specimen was used or improperly clotted serum sample for protein electrophoresis instead of serum?

(a) A bridge between the alpha-1 and alpha-2 globulins
(b) A broad increase in the gamma globulin fraction
(c) A small peak between the beta and gamma globulins
(d) A significantly decreased albumin peak

Answer 7

(c) A small peak between the beta and gamma globulins

Question 8

A biuret method for determining serum total protein is dependent upon:

(a) Amino acid content
(b) Nitrogen content
(c) Number of peptide bonds
(d) Protein precipitation

Answer 8

(c) Number of peptide bonds

Question 9

The Kjeldahl procedure for total protein is based on the premise that:

(a) Proteins are negatively charged
(b) Proteins have similar tyrosine and tryptophan content
(c) The nitrogen content of proteins is constant
(d) The pKa of proteins is the same

Answer 9

(c) The nitrogen content of proteins is constant

Question 10

Which of the following is a method to analyze immunoglobulins?

(a) Biuret method
(b) Evelyn-Malloy method
(c) Immunofixation
(d) Jendrassik-Grof method

Answer 10

(c) Immunofixation

Question 11

Which of the following are correct reference intervals for adults?

(a) Albumin 3.5 - 5.5 g/dL
(b) Gamma Globulins 0.5 - 1.7 g/dL
(c) Total Protein 6.2 - 8.3 g/dL
(d) All Are Correct

Answer 11

(d) All Are Correct

Question 12

Which of the following could be an error in a lab print out that said: Total protein 8.0 g/dL, albumin 8.5 g/dL, gamma globulins 1.0 g/dL?

(a) The albumin is higher than the total protein value
(b) The total protein value is lower than albumin
(c) The albumin + gamma globulins are higher than the total value
(d) All of the above represent errors

Answer 12

(d) All of the above represent errors

Question 13

Why would a total protein value from an ambulatory patient be higher than a patient that laid in bed all day and have the sample drawn for the test?

(a) In reclining/supine patients, water move from intravascular to extravascular spaces
(b) In reclining/supine patients, water move from extravascular spaces to intravascular spaces
(c) Both a and b are true
(d) Neither a or b are true

Answer 13

(b) In reclining/supine patients, water move from extravascular spaces to intravascular spaces

Question 14

What % of protein is made up of nitrogen usually?

(a) 10%
(b) 16%
(c) 20%
(d) 30%

Answer 14

(b) 16%

Question 15

Which of the following is an example of a peptide bond?

Answer 15

(b)

Question 16

Which total protein method requires cupric ions in its reagent system?

(a) Biuret
(b) Folin-Ciocalteu
(c) Kjeldahl
(d) Ultraviolet Absorption

Answer 16

(a) Biuret

Question 17

At pH 8.6, proteins are ________ charged and migrate toward the _______.

(a) Negatively; anode
(b) Positively; cathode
(c) Positively; anode
(d) Negatively; cathode

Answer 17

(a) Negatively; anode

Question 18

What methods are used to measure albumin?

(a) Bromocresyl green
(b) Bromocresyl purple
(c) Nephelometry
(d) All of the above

Answer 18

(d) All of the above

Question 19

Which of the following is true about a refractometer measurement?

(a) A drop of serum is place between a coverslip and a prism on the refractometer
(b) Light is refracted through serum layer, dark area is solute and ~ equal to protein
(c) The method is subject to false positive interferences from azotemia, hyperglycemia, lipemia, icterus, and temp changes
(d) All of the above

Answer 19

(d) All of the above

Question 20

Which of the following are proteins?

(a) Alpha-1-antitripsin, alpha-1-fetoprotein, alpha-2-macroglobulin
(b) Haptoglobin, hemopexin, ceruloplasmin, transferrin
(c) Complement, fibrinogen, C-reactive protein, immunoglobulins
(d) All of the above

Answer 20

(d) All of the above