Quiz 1: Amino Acids and Proteins Flashcards
This structure is an example of:
(a) Amino Acid
(b) Glycine
(c) Hemoglobin
(d) Protein
(a) Amino Acid
Which of the following is an amino acid?
(a) Arachidonic acid
(b) Chylomicron
(c) Glycine
(d) Glycerol
(c) Glycine
Which of the following acute-phase reactant proteins decreases during inflammation?
(a) Alpha-1-antitrypsin
(b) Fibrinogen
(c) Haptoglobin
(d) Transferrin
(d) Transferrin
Which of the following is an acute-phase reactant protein which increases during inflammation?
(a) Alanine
(b) Glycine
(c) Serum Ferritin
(d) Transferrin
(c) Serum Ferritin
Which of the following plasma protein is primarily responsible for maintaining in vivo colloidal osmotic pressure?
(a) Albumin
(b) Alpha-2-Macroglobulin
(c) Fibrinogen
(d) Hemoglobin
(a) Albumin
Which of the following describes a peptide bond?
(a) An amino group and carboxyl group bonded to an alpha-carbon
(b) A double carbon bond between the alpha-carbon and two amino acids
(c) An amino group bonded to a carboxyl group of another amino acid
(d) Two carboxyl groups bonded to the alpha-carbon of an amino acid
(c) An amino group bonded to a carboxyl group of another amino acid
Which of the following conditions would indicate a plasma specimen was used or improperly clotted serum sample for protein electrophoresis instead of serum?
(a) A bridge between the alpha-1 and alpha-2 globulins
(b) A broad increase in the gamma globulin fraction
(c) A small peak between the beta and gamma globulins
(d) A significantly decreased albumin peak
(c) A small peak between the beta and gamma globulins
A biuret method for determining serum total protein is dependent upon:
(a) Amino acid content
(b) Nitrogen content
(c) Number of peptide bonds
(d) Protein precipitation
(c) Number of peptide bonds
The Kjeldahl procedure for total protein is based on the premise that:
(a) Proteins are negatively charged
(b) Proteins have similar tyrosine and tryptophan content
(c) The nitrogen content of proteins is constant
(d) The pKa of proteins is the same
(c) The nitrogen content of proteins is constant
Which of the following is a method to analyze immunoglobulins?
(a) Biuret method
(b) Evelyn-Malloy method
(c) Immunofixation
(d) Jendrassik-Grof method
(c) Immunofixation
Which of the following are correct reference intervals for adults?
(a) Albumin 3.5 - 5.5 g/dL
(b) Gamma Globulins 0.5 - 1.7 g/dL
(c) Total Protein 6.2 - 8.3 g/dL
(d) All Are Correct
(d) All Are Correct
Which of the following could be an error in a lab print out that said: Total protein 8.0 g/dL, albumin 8.5 g/dL, gamma globulins 1.0 g/dL?
(a) The albumin is higher than the total protein value
(b) The total protein value is lower than albumin
(c) The albumin + gamma globulins are higher than the total value
(d) All of the above represent errors
(d) All of the above represent errors
Why would a total protein value from an ambulatory patient be higher than a patient that laid in bed all day and have the sample drawn for the test?
(a) In reclining/supine patients, water move from intravascular to extravascular spaces
(b) In reclining/supine patients, water move from extravascular spaces to intravascular spaces
(c) Both a and b are true
(d) Neither a or b are true
(b) In reclining/supine patients, water move from extravascular spaces to intravascular spaces
What % of protein is made up of nitrogen usually?
(a) 10%
(b) 16%
(c) 20%
(d) 30%
(b) 16%
Which of the following is an example of a peptide bond?
(b)
Which total protein method requires cupric ions in its reagent system?
(a) Biuret
(b) Folin-Ciocalteu
(c) Kjeldahl
(d) Ultraviolet Absorption
(a) Biuret
At pH 8.6, proteins are ________ charged and migrate toward the _______.
(a) Negatively; anode
(b) Positively; cathode
(c) Positively; anode
(d) Negatively; cathode
(a) Negatively; anode
What methods are used to measure albumin?
(a) Bromocresyl green
(b) Bromocresyl purple
(c) Nephelometry
(d) All of the above
(d) All of the above
Which of the following is true about a refractometer measurement?
(a) A drop of serum is place between a coverslip and a prism on the refractometer
(b) Light is refracted through serum layer, dark area is solute and ~ equal to protein
(c) The method is subject to false positive interferences from azotemia, hyperglycemia, lipemia, icterus, and temp changes
(d) All of the above
(d) All of the above
Which of the following are proteins?
(a) Alpha-1-antitripsin, alpha-1-fetoprotein, alpha-2-macroglobulin
(b) Haptoglobin, hemopexin, ceruloplasmin, transferrin
(c) Complement, fibrinogen, C-reactive protein, immunoglobulins
(d) All of the above
(d) All of the above
