Qualitative tests for Carbohydrates Flashcards
1
Q
General Tests for CHO
A
Molisch test and Anthrone test
2
Q
reagent: 𝛼-naphthol + H2SO4
A
Molisch test
3
Q
reagent: anthrone
A
Anthrone test
4
Q
Violet ring at the junction (+) for CHO
A
Molisch test
5
Q
Green color (+) for CHO
A
Anthrone test
6
Q
Reagent: CuSO4 + Na2CO3 + Na citrate
A
Benefict’s
7
Q
Test for reducing sugars
A
- Fehling’s
- Benedict’s
- Barfoed’s (Differentiates mono vs di)
- Tollen’s
- Maillard’s
8
Q
Reagent: CuSO4 + Na-K tartrate
A
Fehling’s
9
Q
Reagent: Ammoniacal AgNO3
A
Tollen’s
9
Q
Reagent: CuSO4 + acetic acid
A
Barfoed’s
10
Q
Brick red ppt (+) For reducing sugars
A
- Fehling’s
- Benedict’s
- Barfoed’s (mono: <5mins vs di: >15mins)
10
Q
Reagent: Amino acids
A
Maillard’s
11
Q
Silver mirror (+) For reducing sugars
A
Tollen’s
12
Q
Brown coloration (+) For reducing sugars
A
Maillard’s
13
Q
Test for ketoses
A
Seliwanoff’s
