Qualitative tests for Carbohydrates Flashcards by doinke zzzz | Brainscape

Q

General Tests for CHO

A

Molisch test and Anthrone test

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Q

reagent: 𝛼-naphthol + H2SO4

A

Molisch test

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Q

reagent: anthrone

A

Anthrone test

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Q

Violet ring at the junction (+) for CHO

A

Molisch test

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Q

Green color (+) for CHO

A

Anthrone test

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Q

Reagent: CuSO4 + Na2CO3 + Na citrate

A

Benefict’s

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Q

Test for reducing sugars

A

Fehling’s
Benedict’s
Barfoed’s (Differentiates mono vs di)
Tollen’s
Maillard’s

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Q

Reagent: CuSO4 + Na-K tartrate

A

Fehling’s

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Q

Reagent: Ammoniacal AgNO3

A

Tollen’s

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Q

Reagent: CuSO4 + acetic acid

A

Barfoed’s

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Q

Brick red ppt (+) For reducing sugars

A

Fehling’s
Benedict’s
Barfoed’s (mono: <5mins vs di: >15mins)

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Q

Reagent: Amino acids

A

Maillard’s

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Q

Silver mirror (+) For reducing sugars

A

Tollen’s

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Q

Brown coloration (+) For reducing sugars

A

Maillard’s

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Q

Test for ketoses

A

Seliwanoff’s

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Q

Test for Pentoses

A

Bial’s and Tollen’s Phloroglucinol

Q

Reagent: Recorcinol, HCl

A

Seliwanoff’s

Q

Reagent: Orcinol, HCl

A

Bial’s

Q

Reagent: Phlolorglucinol

A

Tollen’s phloroglucinol

Q

Blue-green color (+) pentoses

A

Bial’s

Q

Pink / Cherry red (+) ketoses

A

Seliwanoff’s

Q

Red (+) pentoses

A

Tollen’s phloroglucinol

Q

Test for starch and glycogen

A

Iodine test

Q

Reagent: Iodine

A

iodine test

Q

Starch (blue); Glycogen (red)

A

Iodine test

Q

Test for galactose

A

Muric acid test

Q

White insol. crystals (galactaric/mucic acid)

A

Muric acid test

Q

Reagent: Nitric acid

A

Muric acid test

Q

For identification of sugars

A

Osazone test (Kowarsky)

Q

Reagent: Phenylhydrazine

A

Osazone test (Kowarsky)

Q

Yellow-orange crystals of different shapes (+) sugars

A

Osazone test (Kowarsky)