Qualitative Tests for Amino Acids Flashcards
Test for alpha amino acids
Ninhydrin reagent
Violet complex (Ruhemann’s Purple);
Yellow for Proline
Ninhydrin Reagent
Test for Lysine
Schiff’s reagent
Pink-violet (+) lysine
Schiff’s reagent
Test for arginine (guanidine group)
Sakaguchi
Red-orange (+) arginine
Sakaguchi
Old rose
Millon-Nasse
Test for aromaticity (F, W, Y)
Xanthoproteic
Yellow-orange (+) WYF
Xanthoproteic
Tyrosine (phenolic grp)
Millon-Nasse
Tryptophan (indole group)
Hopkins-cole and Bromine water
Pinkish-lavender or violet-alcohol layer (+) W
Bromine water
Violet complex (+) W
Hopkins-cole
Test for cysteine
Fohl’s Test / Lead Acetate and Nitroprusside Test
Test for peptide linkages
Biuret test
Black (+) cysteine
Fohl’s Test / Lead Acetate
Red complex (+) Cysteine
Nitroprusside test
Blue-violet (+) Peptide linkages
Biuret test
Red/purple (+) Y, H
Pauly-Diazo Reaction
Tyrosine, Histidine
Pauly-Diazo Reaction