Qualitative Tests for Amino Acids Flashcards
1
Q
Test for alpha amino acids
A
Ninhydrin reagent
2
Q
Violet complex (Ruhemann’s Purple);
Yellow for Proline
A
Ninhydrin Reagent
3
Q
Test for Lysine
A
Schiff’s reagent
4
Q
Pink-violet (+) lysine
A
Schiff’s reagent
5
Q
Test for arginine (guanidine group)
A
Sakaguchi
6
Q
Red-orange (+) arginine
A
Sakaguchi
7
Q
Old rose
A
Millon-Nasse
7
Q
Test for aromaticity (F, W, Y)
A
Xanthoproteic
8
Q
Yellow-orange (+) WYF
A
Xanthoproteic
9
Q
Tyrosine (phenolic grp)
A
Millon-Nasse
9
Q
Tryptophan (indole group)
A
Hopkins-cole and Bromine water
10
Q
Pinkish-lavender or violet-alcohol layer (+) W
A
Bromine water
10
Q
Violet complex (+) W
A
Hopkins-cole
11
Q
Test for cysteine
A
Fohl’s Test / Lead Acetate and Nitroprusside Test
11
Q
Test for peptide linkages
A
Biuret test
