Qualitative Tests for Amino Acids Flashcards

1
Q

Test for alpha amino acids

A

Ninhydrin reagent

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2
Q

Violet complex (Ruhemann’s Purple);
Yellow for Proline

A

Ninhydrin Reagent

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3
Q

Test for Lysine

A

Schiff’s reagent

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4
Q

Pink-violet (+) lysine

A

Schiff’s reagent

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5
Q

Test for arginine (guanidine group)

A

Sakaguchi

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6
Q

Red-orange (+) arginine

A

Sakaguchi

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7
Q

Old rose

A

Millon-Nasse

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7
Q

Test for aromaticity (F, W, Y)

A

Xanthoproteic

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8
Q

Yellow-orange (+) WYF

A

Xanthoproteic

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9
Q

Tyrosine (phenolic grp)

A

Millon-Nasse

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9
Q

Tryptophan (indole group)

A

Hopkins-cole and Bromine water

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10
Q

Pinkish-lavender or violet-alcohol layer (+) W

A

Bromine water

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10
Q

Violet complex (+) W

A

Hopkins-cole

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11
Q

Test for cysteine

A

Fohl’s Test / Lead Acetate and Nitroprusside Test

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11
Q

Test for peptide linkages

A

Biuret test

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12
Q

Black (+) cysteine

A

Fohl’s Test / Lead Acetate

13
Q

Red complex (+) Cysteine

A

Nitroprusside test

14
Q

Blue-violet (+) Peptide linkages

A

Biuret test

15
Q

Red/purple (+) Y, H

A

Pauly-Diazo Reaction

15
Q

Tyrosine, Histidine

A

Pauly-Diazo Reaction