Purpose of Lab Techniques

Question 1

PCR?

Answer 1

Amplify a desired fragment of DNA

Question 2

Agarose gel electrophoresis?

Answer 2

Separate size of PCR products

Question 3

Southern blot?

Answer 3

DNA

Question 4

Northern blot?

Answer 4

RNA

Question 5

Western blot?

Answer 5

Protein

Question 6

Southwestern blot?

Answer 6

DNA-binding proteins (e.g. transcription factors)

Question 7

Microarray?

Answer 7

Profiling gene expression–studying disease, txs, genotyping, forensic analysis, predisposition to dz, cancer mutations, genetic linkage analysis

Question 8

ELISA?

Answer 8

Test Ag-Ab reactivity–to see if a particular Ab is present in blood

Question 9

Indirect ELISA?

Answer 9

Uses test Ag to see if specific Ab is present

Question 10

Direct ELISA?

Answer 10

Uses test Ab to see if specific Ag is present

Question 11

FISH?

Answer 11

Specify gene location and direct visalization of microdeletions (when deletions are too small for karyotyping)

Question 12

Knock out mouse?

Answer 12

Take a gene out

Question 13

Knock in mouse?

Answer 13

Insert a gene

Question 14

Cre-lox system?

Answer 14

To study a gene whose deletion causes embryonic death

Question 15

RNAi?

Answer 15

Used to knock down gene expression

Question 16

Karyotyping?

Answer 16

Dx of chromosomal imbalances (autosomal trisomies, sex Chr disorders)

Question 17

Steps of PCR?

Answer 17

Denaturation (heat up), annealing of DNA primers, elongation by DNA polymerases