Pulmonary Biochemistry Week 3 Flashcards

1
Q

True/False: Respiratory acidosis/alkalosis are always caused by abnormal function of the lung

A

FALSE - often but not always. For example, a person can have respiratory acidosis (increased PaCO2) but lungs are normal

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2
Q

On the davenport diagram, the x-axis represents ____ and the y-axis represents _____ therefore any point on the graph is a solution of the Henderson equation. Overall, the Davenport diagram tells you how blood will respond to changes in ______

A

pH, [HCO3-], PaCO2

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3
Q

Davenport diagrams reflect the presence of _______ buffers in the blood

A

non-volatile [presence of Hb mitigates increase in h+ caused by shifts in bicarbonate buffer system]

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4
Q

On a davenport diagram, a steeper buffer line of blood [the line reflecting the changes paCO2 have on pH and HCO3-], would indicate what?

A

more non-volatile buffering power [basically, with greater changes in PaCO2 there are slightly greater changes in HCO3- but lesser changes in pH so line is steeper]

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5
Q

Describe three non-pulmonary causes of acute respiratory acidosis

A

disruption in neural linkage driving breathing, central nervous system depression[drug OD, anesthesia], external enviro preventing normal breathing[heavy weight]

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6
Q

Name 5 pulmonary causes of acute respiratory acidosis

A

upper airway obstruction, severe asthma attack, COPD, severe pneumonia, severe pulmonary edema

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7
Q

Respiratory acidosis/alkalosis is caused by a pathological change in _____ whereas metabolic acidosis or alkalosis is caused by a pathological change in ______

A

PaCO2, [HCO3-]

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8
Q

With a decrease in CO2 in which direction will the equilibrium shift. How do Hb buffers play a role.

For reference, the rxns:
CO2 H+ + HCO3-
H+ + Hb H+Hb

A

The equilibrium will shift towards the left to produce more CO2. H+ will be lost, but this will be mitigated by Hb releasing H+.

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9
Q

______ is caused by an increase in central drive to breathe

A

Respiratory alkalosis

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10
Q

True/False: For respiratory alkalosis to occur, the entire neuromuscular chain for breathing must be intact

A

True

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11
Q

What are four conditions that act as abnormal ventilatory stimuli to increase central drive to breathe?

A

1) arterial hypoxemia or hypoxia; 2) direct stimulation of pulmonary mechanoreceptors and chemoreceptors by lung dz; 3) chemical or physical factors that stimulate the medullary respiratory center; 4) psych factors

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12
Q

True/False: Most common pulmonary diseases can lead to respiratory alkalosis

A

True

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13
Q

Describe the three conditions of acute asthma that act as abnormal ventilatory stimuli to increase breathing

A

Stimulation of sense receptors; anxiety (psych); hypoxemia

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14
Q

Describe the three conditions of pulmonary embolus that act as abnormal ventilatory stimuli to increase breathing

A

stimulation of sense receptors; pain (psych); hypoxemia

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15
Q

Describe the four conditions of bacterial pneumonia that act as abnormal ventilatory stimuli to increase breathing

A

inflammatory debris [direct stimulation of mechano/chemo receptors]; fever [direct stimulation of mechano/chemoreceptors]; anxiety [psych], and hypoxemia and hypoxia

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16
Q

How does hypoxemia cause respiratory alkalosis?

A

The peripheral chemoreceptors in the carotid and aortic bodies begin to drive breathing when PaO2 falls below ~60mmHg [so then CO2 blown off]

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17
Q

The same diseases that cause respiratory alkalosis can cause respiratory acidosis. What two factors would allow the transition to acidosis to occur?

A

If the disease progresses to a point of severity when MUSCLE STRENGTH IS WEAKENED AND/OR LOAD IS INCREASED enough to “tip the balance”

Load=airflow resistance, lung stiffness, ventilatory requirement
Strength=central drive, neural linkage, resp muscles [4 abnormal ventilatory stimuli]

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18
Q

In end-stage liver disease, ________ is quite common. This disease leads to intrapulmonary shunting that causes low V/Q regions. Pt’s will be _____ and have a high A-a gradient

A

hepatopulmonary syndrome, hypoxemic

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19
Q

Respiratory acidosis is caused by _______ via __________ whereas respiratory alkalosis is caused by ________ via _________

A

hypercapnia, alveolar hypovenilation, hypocapnia, alveolar hyperventilation

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20
Q

Describe what occurs when a strong acid is added to a blood sample [open to chamber containing CO2 gas at 40mmHg]

Reference rxn: CO2 H+ + HCO3-

A

Adding H+ will shift equilibrium to left. HCO3- consumed to consume of of added acid. PCO2 will remain at 40mmHg.

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21
Q

Describe what occurs when a strong base is added to a blood sample [open to chamber containing CO2 gas at 40mmHg]

Reference rxn: CO2 H+ + HCO3-

A

Strong base [A-] will react with H+ to form HA. Therefore, H+ used up. To replace H+, equilibrium shifted to the right. HCO3- increases. PCO2 remains at 40mmHg.

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22
Q

What are two main causes of hypobicarbonatemia [which leads to metabolic acidosis]?

A

increase in EAP, reduced net excretion due to renal defects

[increase in EAP can be due to derangements in gut function, derangements in metabolism, exogenous intoxicants]

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23
Q

Describe how diarrhea or laxative abuse is a gastrointestinal cause of metabolic acidosis.

A

Diarrhea and laxative abuse lead to an increase in stool volume which means a lot of HCO3- is lost from the body. The lower gut cells secrete more HCO3- to replace the lost HCO3- which leads to increased H+ secretion into the blood. The increased H+ secretion into the blood leads to blood HCO3- being consumed.

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24
Q

Metabolic acidosis is caused by an imbalance between _______ and ______

A

organic acid production, consumption

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25
Normally, there is an incomplete metabolism of carbs and lipids. Carbs and lipids are metabolized to organic acids, but do not finish complete metabolism to A- and H+. What is needed to complete this metabolism?
Oxygen
26
What happens during hypoxia in regards to carb/lipid metabolism?
Carb lipid metabolism is incomplete so there is a build up of organic acids [along with protons] examples -metabolic acidosis (strenuous exercise with volume depletion) and ketoacidosis (type I diabetes)
27
______ and _____ are two alcohols that get metabolized to acids and therefore are exogenous causes of metabolic acidosis
methanol, ethylene glycol [essentially dissociate into A- + H+ and will be EAP like]
28
How does vomiting or nasogastric drainage cause metabolic alkalosis?
H+ are being lost which means that upper GI is generating more H+ --> upper GI is secreting more HCO3- into the blood --> initially, body will dump HCO3- via urine to keep pH normal --> if vomiting/nasogastric drainage continues, will result in metabolic alkalosis
29
What two mechanisms are required for metabolic alkalosis to occur?
A generation mechanism [such as vomiting] and a maintenance mechanism [such as increase in renal threshold for HCO3- spillage]
30
Virtually all causes of metabolic alkalosis present with hypokalemia or at least a low-normal K+. Why?
1) H+ moved out of cells to relieve alkalosis which means K+ moves into cells, reducing K+ concentration in plasma 2) Volume depletion increases aldosterone levels --> more Na+ reabsorbed and K+ secreted --> more K+ loss via kidneys
31
What are the three main factors responsible for maintenance of metabolic alkalosis?
volume depletion, hypokalemia, aldosterone excess
32
What are the two mechanisms by which loop diuretics and thiazide cause alkalosis?
1) Loop diuretics and thiazide block Na/K/Cl and Na/Cl transporters in the nephron. Therefore there is more Na+ and Cl- which leads to saline diuresis. Later in the nephron, some Na+ is reabsorbed which increases K+ secretion. This drives the nephron in a later segment to reabsorb K+ which leads to H+ secretion. This results in increased H+ loss. I 2) Greater K+ excretion makes the body hypokalemic, so K+ moves out of the cells to compensate which drives H+ into the cells, decreasing the H+ plasma concentration.
33
True/False: In treatment of edema with loop diuretics and thiazide, virtually all of HCO3- contained in the edema fluid is retained leading to alkalosis
True
34
Explain the rule of thumb for acute respiratory acidosis in a simple acute disturbance.
pH down by 0.07 and HCO3- up by 1mM = for a 10mmHg change in PaCO2, the condition is acute and has not been compensated
35
Explain the rule of thumb for acute respiratory alkalosis in a simple acute disturbance
pH up by 0.08 and HCO3- down by 2mM = for a 10mmHg change in PaCO2, the condition is acute and has not been compensated
36
Does a patient who has a pH of 7.26, a PaCO2 of 60mmHg, and an [HCO3-] of 26 meet the rule of thumb for acute respiratory acidosis?
Yes
37
In terms of acidosis/alkalosis, what does compensation mean?
change in [HCO3-] or PaCO2 that occurs as a result of the primary disturbance
38
True/False: In acidosis/alkalosis, compensation is never complete, so pH will only approach 7.4
TRUE
39
Why are there acute and chronic respiratory alkalosis/acidosis categories, but no distinction for metabolic acidosis/alkalosis?
Respiratory compensation for metabolic conditions occurs very quickly (basically, immediately)
40
how long does chronic compensation (via renal system) take for a) respiratory acidosis; b) respiratory alkalosis
4 days; 8 days [highly variable]
41
In respiratory acidosis, how long does mitigation of pH change by equilibration of body buffers take?
10 minutes
42
Using the rules of thumb, how can you differentiate between chronic and acute respiratory acidosis/alkalosis?
For a 10mmHg change in PCO2, the pH will change more in acute [0.08] than in chronic [0.03]
43
A patient with severe COPD presents with a pH of 7.38, PaCO2 of 70mmHg, and [HCO3-] of 40mM. What is the likely acid-base disturbance?
The patient does not have acute respiratory acidosis - change in HCO3- is due to primary disturbance PCO2. The renal system has had time to compensate by increasing plasma bicarbonate, which returned pH back toward normal.
44
A patient with a history of methanol ingestion has a pH of 7.29, HCO3- of 12, and PaCO2 of 26. What is the acid-base disturbance?
pH is very low, HCO3- is low, and PaCO2 is low. Pt has metabolic acidosis [which is rapidly compensated by respiratory hyperventilation which is why PaCO2 is low].
45
In metabolic acidosis, what is the primary change and rapid compensated change?
Primary change is fall in HCO3-; rapid compensatory change is fall in PaCO2
46
In metabolic alkalosis, what is the primary change and the compensatory change?
Primary change is rise in HCO3-; compensatory change is rise in PaCO2
47
If pt has pH of 7.29, HCO3- of 14, PaCO2 of 30, what is acid base disturbane
metabolic acidosis bc bicarb low - note that PaCO2 is lower than nl so the metabolic acidosis has been rapidly compensated
48
If pt has pH of 7.57, HCO3- of 42, PaCO2 of 47, what is acid base disturbance?
metabolic alkalosis - note that PaCO2 is higher than normal reflecting compensation for high HCO3-
49
If pt has pH of 7.57, HCO3- of 18 and PaCO2 of 18, what is acid base disturbance
acute respiratory alkalosis - fit rule of thumb for a 10mmHg drop in PaCO2, the pH goes up by 0.08 units
50
If pH is 7.60, HCO3- is 32, and PaCO2 is 24, what this the acid base disturbance
mixed
51
A severely bulimic woman has pH 7.51, HCO3- of 38, PaCO2 of 49, what is the acid base disturbance?
Pt has metabolic alkalosis with respiratory compensation [this is why PaCO2 is elevated] Pt fits the rule of thumb for metabolic alkalosis - PaCO2 up by 0.5 to 1mM for each 1mM rise in HCO3-
52
What are the four electrolytes that are routinely measured and what are normal concentrations?
Na+ (140mM), K+ (4mM), Cl- (100mM), bicarb (24-30mM)
53
What three pieces of data that are relevant to acid-based disturbances do venous electrolytes provide?
CO2 or total CO2 (often called bicarb); anion gap; potassium concentration
54
What is the anion gap? [think equation]
[Na+]- ([Cl-] + [CO2])
55
How is total venous CO2 measured with arterial blood gas?
ABG measures [HCO3-] and is usually calculated from H-H or henderson eqn after measuring pH and PaCO2
56
How is total venous CO2 measured with venous electrolytes?
labeled CO2 or total CO2 instead of bicarb - measured in mM instead of mmHg bc not a measure of total gaseous CO2 dissolved. This equals close to the CO2 dissolved + [HCO3-]
57
Venous CO2 is ______ than arterial [HCO3-]
greater; normal venous CO2 range 24-30mM; normal arterial [HCO3-] range 22 to 26mM
58
What are the unmeasured anions that make up a normal anion gap?
sulfate, phosphate, organic anions, protein
59
The ____ is used in anion gap calculation whereas the _____ is used in rules of thumb for acid base disturbance
venous CO2 (total CO2); arterial HCO3- (true bicarb)
60
If a patient has a pH of less than 7.35 and a bicarb less than 24, and the anion gap is less than 12, what type of acid-base disturbance do they have?
non-gap acidosis
61
What three disorders account for most cases of anion gap metabolic acidosis?
ketoacidosis (alcoholic or diabetic), lactic acidosis), renal failure
62
_____ and _____ are the predominant anions in ketoacidosis
acetoacetate, beta hydroxybutyrate
63
______ is the predominant anion in lactic acidosis
lactate
64
Sulfate, phosphate, urate, hippurate are the premoninant anions in _______
renal failure [normal excreted by the kidney]
65
_______ is a product of anaerobic metabolic and increased with ______
lactic acid; hypoxia
66
what are the two most common causes of lactic acidosis?
circulatory failure (cardiogenic shock) and sepsis (septic shock)
67
What three conditions would be produce an overproduction of ketoacids?
diabetic (usually type1); starvation; alcoholic (drinking and vomiting binges)
68
What are the three disorders that account for most cases of non-anion gap metabolic acidosis?
diarrhea, renal tubular acidosis, renal failure (chloride from increased chloride retention)
69
True/False: Renal failure is the most common cause of non-anion gap metabolic acidosis?
FALSE - diarrhea
70
What are the two sites of heme biosynthesis?
erhythroid cells (~85%) and hepatocytes (~15%)
71
True/False: heme and iron metabolism are tightly coupled
TRUE DAT
72
Heme is composed of ____ and _____
Fe2+, protoporphyrin IX
73
Hypoxia stimulates eryhthropoiesis which requires ____, ____ and _____
iron, Hb, heme
74
In the bone marrow, multipotential hematopoietic stem cells give rise to ______ that then divide into _____
erythroid progenitors, erythroblasts
75
After the _______, the erythyroid cell leaves bone marrow as a ______
expulsion of the nucleus, reticulocyte
76
What organelles do reticulocytes lose? [nucleus already lost]
mitochondria, ribosomes
77
Mature erythrocytes participate in what 3 metabolism processes?
glycolysis, pentose phosphate shunt, reductive capacity
78
Describe how hypoxia and normoxia affects HIF-1 postranslational regulation
If hypoxic, HIF-1 transcription factor is phosphorylated which allows translocation to nucleus where it causes transcriptional activation of oxygen-regulated gene expression. If normoxic, HIF-1 is ubiquitylated then degraded via proteasome
79
The enzyme _______ is involved in the "oxygen sensing mechanism" that adds an OH to HIF-1 if in normoxic environment
prolyl hydroxylase
80
What are the four HIF-1 target genes involved in erythropoiesis and iron metabolism?
EPO, transferrin, transferrin receptor, ceruloplasmin
81
______ is involved in iron uptake
transferrin receptor
82
______ is involved in iron oxidation and release of iron from stores
ceruloplasmin
83
_____ is involved in iron transport
transferrin
84
What does HIF-1 stand for and what is its overall function as a transcriptional regular?
Hypoxia inducible factor; regulates genes that promote cell survival under ischemic conditions
85
HIF-1 is a crucial regulator of ______, which synchronizes cell responses, Hgb, and iron metabolism and other metabolic pathways, assuring ________ to satisfy body needs
erythropoietesis, red cell production
86
____ is a protein hormone produced by the ___ which binds receptors in the _____ where it stimulates production of RBC's
EPO, kidney, bone marrow
87
EPO is used to treat certain forms of anemia such as those due to _____
chronic kidney failure
88
Since EPO accelerates erythrocyte production is also increases ____
CaO2 (O2 carrying capacity)
89
What is blood doping and how is it accomplished?
Artificially increasing RBC to improve athletic performance, previous was accomplished via transfusion and now via EPO injection
90
How is blood doping dangerous?
increases blood viscosity [blood can sludge and clog capillaries leading to stroke or heart attack] and xs prolif of RBC's can cause polycythemia
91
Why is blood doping so difficult to detect?
Recombinant EPO is identical to endogenous. Can be differentiated by HCT, retic count, soluble transferrin receptor content and concn of b-globin mRNA.
92
What is the rate limiting step of hepatic heme synthesis and where does it occur?
FIRST STEP - delta-aminolevulinate synthase (delta-ALA synthase) catalyzed step, occurs in mitochondria
93
ALAS-N synthesis is inhibited by _____ and induced by _____
heme, compounds that increase hepatic cytochrome P450 synthesis (needs heme)
94
What variant of ALAS is found in the liver and which is found in erythroid cells?
ALAS-N in liver (ALAS non-specific or ALAS-1); ALAS-2 found in erythroid cells
95
True/False: Like ALAS-1 in liver cells, ALAS-2 in erythroid cells is limited by heme
FALSE - heme does not repress ALAS-2 expression in erythroid cells - thus regulation is different in erythroid cells than liver
96
Describe heme catabolism. What is the rate limiting step?
heme --> biliverdin --> bilirubin Heme converted to biliverdin via heme oxygenase (RLS) Biliverdin converted to bilirubin via biliverdin reductase
97
Heme is converted to bilirubin by _______
reticuloendothelial cells
98
How does bruising exemplify heme catabolism?
Purple color of heme in hematoma (bruise) converted to yellow pigment of bilirubin
99
Bilirubin is transported to the _____ bound to plasma _____ where the majority of further metabolism occurs
liver, albumin
100
In the liver's ______, bilirubin uptake occurs then bilirubin is conjugated to ______ and secreted in _____
parenchymal cells, glucuronate, bile
101
Bilirubin is readily excreted in bile as soluble form ________ and then further reactions occur via bacteria in the ____
bilirubin diglucorinide, colon
102
Hyperbilirubinemia causes _____
jaundice
103
What three situations could cause hyperbilirubinemia?
damaged liver that cannot excrete normal amount of bilirubin; overproduction of bilirubin; obstruction of excretory ducts of liver
104
_____ or ____ bilirubin is transported to liver where it is ______ to ______
indirect, unconjugated (UN-IN), conjugated, direct
105
What would cause an increase in unconjugated bilirubin vs conjugated bilirubin?
unconjugated - overproduction, impaired uptake, impaired conjugation conjugated - decreased excretion into the bile ductules or backward leakage
106
What is the most common cause of unconjugated hyperbilirubinemia? What are the dangers and how is it treated?
Physiologic jaundice - common in babies due to immature hepatic system that cannot conjugate bilirubin (bilirubin-UGT activity reduced --> reduced synth of UDP-glucoronic acid) Dangers - unconjugated bilirubin can penetrate blood brain barrier --> toxic encephalopathy (kernicterus) or mental retardation TX - blue light (phototherapy) - promotes hepatic excretion of unconjugated bili by converting it into other derivatives that are excreted in bile
107
True/False- O2 sensing mechanism is present in all cells
True
108
What coordinates regulation of O2 transport?
erythropoiesis and iron metabolism
109
True/False: Iron has very low bioavailability because most commonly forms are iron-oxide and metallic iron which have little use to organisms
True
110
Oxygen is normally in a _____ state meaning it has _______. Oxygen can react with iron in one electron _______
triplet, unpaired electrons, red ox reactions
111
Iron can easily be _____ and _____ from Fe2+ to Fe3+
oxidized and reduced
112
Iron plays a role in oxygen ____ and _____ via ______ and _______
transport, storage, Hgb, methoglobin
113
_______ in the electron transport chain contain both heme and iron-sulfer centers
cytochromes
114
_____ involved in amino acid metabolism and ______ used in the inflammatory response both contain iron
monooxygenases, dioxygenases
115
Iron and O2 generate ______ and there are many mechanisms in place to avoid production of _____
free radicals; xs reactive oxygen metabolites
116
_____, ______, and ______ sequester iron and change environment of iron to avoid unwanted side effects
binding proteins, protoporhyrins, and Fe-S centers
117
How does iron and oxygen generate free radicals?
Iron gives up an electron to oxygen --> Fe3+ and superoxide anion (O2-)
118
____ is the iron from meat, wherea's ______ is the iron found in mother's milk
heme, lactoferrin
119
Where in the body is iron absorbed?
upper small intestine
120
Total body content of iron is ______ and system usually has enough due to reclamation by _______
3-5 grams, reticuloendothelial system
121
True/False: Function of the diet is to keep iron concentration of the body topped off
True
122
How can excessive needs of iron be met by the body?
depletion of Fe-ferritin stores and increased iron uptake
123
About 1mg of iron is lost per day. What are the 4 main mechanisms by which iron is lost?
through intestine (occult blood loss), sloughed enterocytes, biliary secretions, skin cells [also menstruation, blood donation, hemorrhage (nosebleeds), pregnancy]
124
Heme-iron is taken up by a heme transporter (HT) protein and once in cell _______ splits iron from heme
heme dioxygenase
125
True/False: Gut environment promotes Fe2+ to Fe3+
True
126
Fe3+ is reduced to Fe2+ by _______ at ______
ferric reductace, duodenal cytochrome b
127
After reduction, Fe2+ is transported by ___ across ____ of the enterocyte, into the enterocyte
DMT1, apical
128
Once in the enterocyte, _______ aided by hephaestin transports Fe2+ to interstitial fluid then to plasma. In the process, hephaestin converts Fe2+ to ____
ferroportin (fp), Fe3+
129
After entering the plasma, plasma Fe3+ binds to ______ to form ______
apo-transferrin, transferrin
130
True/False: SOme Fe2+ is stored as Fe3+-ferritin in enterocyte
True
131
______ is the major storage protein of iron and contains _____ per protein molecule in the form of Fe3+ oxide-hydroxide
ferritin, 4500 irons
132
What is the aggregated form of Fe3+ oxide-hydroxide (ferritin storage molecule) called? This is seen in iron overload
hemosiderin (partially degraded)
133
True/False: Serum ferritin level is the most convenient lab test to estimate iron stores
True
134
Why is transferrin used as a carrier of iron in plasma?
Fe(III) is insoluble at physiological pH so transferrin needed to bind iron(III) to avoid precipitation in plasma
135
True/False: Essentially all plasma iron is bound to transferrin because free iron is damaging at high concentrations
FALSE - free iron is damaging even at low concentrations
136
Transferrin carries ___ Fe3+ atoms at a time and the % saturation of transferrin with iron usually around ___
2, 33%
137
There are about _____ transferrin receptors on the cell surface of a developing erythroblast
300K-400K
138
Transferrin binding to receptor is modulated by _____
HFE
139
Once transferrin binds to transferrin receptor, the entire complex is endocytosed. The interior of the endosome is then acidified to release _____ via ferric reductase which is then taken up by the mitochondria and used by ______ to load iron into heme
Fe2+, ferrochetolase
140
After losing its Fe in the endosomal vesicle, what happens to transferrin?
apotransferrin is recycled back to the plasma to bind more Fe3+
141
True/False: transferrin receptors return to the membrane after being endocytosed
true
142
What is the job of the iron regulatory proteins?
stabilize transferrin receptor mRNA
143
The _____ is the primary site of iron storage and is the master control organ.
Liver
144
______ is a peptide produced by the liver to inhibit processes that put iron into blood and may be a master regulator of iron homeostasis.
Hepcidin
145
How does hepcidin inhibit liver processes ?
When hepicidin concentration is high, hepcidin directly interacts with ferroportin and causes its internalization, therefore trapping iron in enterocytes, macrophages and hepatocytes
146
What two conditions stimulate hepcidin production?
inflammation and iron overload
147
True/False: Even at low concentrations, hepicidin significantly inhibits iron from entering the plasma
FALSE - at low concentrations, iron enters plasma at high rate
148
In duodenal epithelial cells (AKA villus), dietary iron imported via _____. Enters plasma via _____ that is regulated by ______.
DMT1, ferroportin, hephaestin
149
In liver parenchymal cells, iron imported via _____ and enters plasma via _______ that is regulated by ____.
transferrin receptor, ferroportin, ceraluplasmin
150
In reticuloendothelial macrophages (which recycle iron), iron imported via ____ and enters plasma via _____ which is regulated by _____
phagocytosis of RBC, ferroportin, ceraluplasmin
151
Iron imported into RBC precursors via ______ and then enters plasma via ____
transferrin receptor; being in an RBC that is eventually phagocytosed [TRICK QUESTION]
152
Iron transported into an RBC precursor is converted to a Hb-heme complex via _____
mitochondrial ferrochetolase
153
Which of the following do not contain a ferroportin protein for iron export in their membrane? a) liver parenchymal cell b) RBC precursor c) reticuloendothelial macrophages d) duodenal epithelial cells
B
154
How does iron regulate expression of FE-binding proteins such as ferritin? [think IRE and IRP]
The ferritin coding sequence mRNA has an iron response element (IRE) on its 5' end. At low iron concentration, the IRE is bound by iron regulatory proteins and IRP has a 3Fe-4S sulfur center. In this configuration, translation of iron is blocked. When iron levels are high, iron will bind to IRP and convert iron sulfur center to a 4Fe-4S center. This changes the conformation so IRP no longer binds tightly to IRE on 5' end. WHen there is no IRP bound, translation proceeds and you will get high ferritin synthesis.
155
Why is ferritin not needed when iron is low?
because ferritin is a storing protein, so if iron is low, won't need to store as much
156
In _______, there is an inappropriate increase in _____ iron absorption which results in deposition of excessive amount of iron in _____ with eventual tissue damage and impaired function of organs
hemochromatosis, intestinal, parencyhmal cells
157
When xs iron is deposited in parenchymal cells, it is deposited in the form of ______
hemsodiren (aggregated ferritin)
158
What are the 3 primary affected tissues in hematochromatosis?
liver-cirrhosis, pancreas-diabetes, skin-bronze (bronze diabetes)
159
The primary cause of hemochromatosis is a ______ allele that contains a _____ gene that associates with beta-2-microglobulin and forms complex with transferrin receptor
autosomal recessive, MHC1
160
True/False: The genetic form of hematochromatosis is the primary cause of iron overload.
True
161
How is the overload in hemochromatosis reflected and when?
serum ferritin concentrations, start to increase in later teens [increase in ferritin parallels the severity of the disease]
162
True/False: Serum ferritin contains little or no iron
FALSE
163
True/False: Tissue concentrations of ferritin are much higher
true
164
What is the most common cause of poisoning deaths in children less than 6 years old?
acute accidental iron poisoning [most often vitamins]
165
What is the treatment for acute iron poisoning?
strong laxative and IV deferoxamine mesylate
166
How does IV deferoxamine mesylate (desferal) treat iron poisoning?
binds iron and excreted in urine
167
Why is iron poisoning toxic?
Serum free iron is not bound to transferrin and is highly toxic because it can cause lipid peroxidation which damages blood vessels (changing BP) and damage mitochondrial membranes (causing problems with O2 handling)
168
What is the most common amount of anemia?
iron deficiency
169
True/False: iron deficiency results from blood loss in most adults
True
170
True/False: about 5% of premenopausal women are iron deficiency anemic
True
171
What are three characteristics of iron deficiency anemia?
weakness, pallor, exercise intolerance
172
What is the amount of blood in the body of an average adult? During donation, how much iron is lost?
10 pints; 200-250mg
173
Donating one unit (equal to one pint) of blood per year is equivalent to an increased requirement for iron of _____ per day
0.65 mg
174
True/False: If males donated one unit per year, that would halve the serum ferritin level
True
175
True/False: Males can donate 2-3 times per year without appreciable incidence of iron deficiency, and women can only donate about half that amount, which makes them more susceptible
True
176
The first step of the heme synthesis pathway involves _____ and ______
succinyl CoA, glycine
177
2) after d-ALA is formed, two molecules are dehydrated via ______ to form _______, a pyrrole precursor
ALA dehyrase, porphobilinogen
178
3) Porphobilinogen is then catalyzed by uropophyrinogen ____ and ____ synthase in subsequent steps to yield ________, and in the process lose ___
I, III, uroporyphyrinogen III (that was a gimme), 4 ammonium ions
179
4) The uroporphyrinogen III contains ____ and _____ sidegroups that are important in helping the heme insert into the hydrophobic cleft of the ______
acetate, proprionyl
180
5) Uroporphyrinogen III is then converted to _____ via uroporphyrinogen decarboxylase in a reaction that loses _____. In this process, the acetate side groups are converted to _____
coproporphyrinogen III, 4 CO2, methyl
181
6) Coproporphyrinogen III is then converted to _______ via coproporphyrinogen oxidase in a process that loses _____. In this process, some of the proprionyl side groups are converted to _____
protoporyphyrinogen IX, 2 CO2, vinyl
182
7) The protoporphyrinogen IX molecule is then converted to ________ via protoporphyrinogen oxidase. What other major change takes place?
protoporphyrin IX. Double bonds are added to the rings so the molecule becomes red in color [previously colorless]
183
8) Protoporphyrin IX then reacts to form heme via _____. ____ is coordinated to the pyrrole nitrogens when added into the ring.
ferrochetolase, Fe2+
184
Which enzymes involved in the heme synthesis pathway are found in the cytosol? Which are found in the mitochondria?
1, 6, 7, 8 [ALA-synthase, coprop oxidase, photopor oxidase, ferrochetalase] are in the cytosol The rest are found in the mitochondria
185
True/False: The heme biosynthesis pathway begins in the cytosol and ends in the mitochondria
FALSE: begins in mito, then in cytoplasm, begin in mito
186
Individuals with low activities of ALA-synthase (enzyme 1) develop ______
anemia
187
_____ are genetic or acquired abnormalities in heme synthesis pathway that develop due to depression of enzyme ____ activity
porphyria, 3-8
188
Mutations in DNA leading to accumulation of ALA and PBG (first two steps) and/or decrease in heme in cells and body fluids would lead to what symptoms?
neuropsychiatric signs and symptoms
189
Mutations in DNA leading to accumulation of porphyrinogens in skins and tissues would lead to what symptoms? How?
Photosensitivity. Porphyrinogens would spontaneously oxidase to porphyrins in the light. This causes lesions and discomfort.