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RUSVM Bacteriology > Pseudomonas and Burkholderia > Flashcards

Pseudomonas and Burkholderia Flashcards

1
Q

Pseudomonas Characteristics

A

Obligate aerobic
Motile rods (flagella)
Most saprophytic
Mainly in water

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2
Q

P. Aeruginosa Characteristics

A 
Facultative symbiotic
Facultative pathogenic (usually secondary)
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3
Q

P. Aeruginosa - Found in

A

Soil
Water
On Plants

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4
Q

P. Aeruginosa - Growth Characteristics

A

Easy grower
Large colonies
Sweet odor
Blue-green pigment

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5
Q

P. Aeruginosa is Oxidase

A

Positive

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6
Q

P. Aeruginosa is Lactose

A

Negative

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7
Q

P. Aeruginosa - Infections

A

Evironmental origin
High natural resistance
Can adapt to humid hospital environments (tubes)

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8
Q

P. Aeruginosa - Virulence Factors

A 
Adhesion (fimbriae)
LPS Endotoxin
Exotoxin
Biofilm
Capsule
Iron acquisition
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9
Q

P. Aeruginosa - Affects

A

Cold blooded animals

Secondary infections in mammals and birds

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10
Q

P. Aeruginosa - Predisposing Factors

A

Drinking water
Humidity
Reduced immunity

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11
Q

P. Aeruginosa in Cats and Dogs

A

Skin infections
Cystitis
Otitis externa
Purulent process - blue green pus

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12
Q

P. Aeruginosa in Rodents

A

Pneumonia
Septicemia
High mortality

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13
Q

P. Aeruginosa in Rabbits

A

Skin infection - moist dermatitis

Sporadic pneumonia

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14
Q

P. Aeruginosa in Horses

A

Metritis-vaginitis

Keratitis conjunctivitis - secondary infection

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15
Q

P. Aeruginosa Causes Mastitis in

A

Bovine, sheep, goat

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16
Q

P. Aeruginosa Mastitis

A

Acute with High Mortality

Origin - environment, teat injector

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17
Q

P. Aeruginosa in Reptiles

A

Necrotic stomatitis
Pneumonia
Septicemia
Secondary to poor housing

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18
Q

P. Aeruginosa - psittaciformes in Parrots/Parrakeets

A 
Secondary infection
conjunctivitis
Rhinitis
Pneumonia
Airsacculitis
Enteritis
19
Q

P. Aeruginosa - Galliformes

A

Water supply
Oviduct of turkeys
Contamination of eggs

20
Q

P. Aeruginosa - Treatment

A

Take away cause of infection
Fluoroquinolones, Aminoglycosides, polymixin
Most animals die

21
Q

Pseudomonas spp.

A

Cold blooded animals (reptiles, fish)

Skin ulcus/septicemia

22
Q

Burkholderia Characteristics

A

Aerobic
Rods
Catalase positive

23
Q

B. Mallei: Glanders

A 
BSL3 agent
Zoonotic
Equine pathogen
Must be reported
Mainly in Asia and S. American
24
Q

B. Mallei: Glanders - Symptoms

A

Nodules and ulcers, pyogranulomatous
Orchitis
Skin, resp tract
Variability in severity

25
Q

Glanders - Virulence

A

Capsule

LPS

26
Q

Glanders - Transmission

A

Ingestion - feed, water
Inhalation
Wounds

27
Q

Glanders - Pathogenesis

A

Chronic, debilitating

pharynx/skin –> lymph and blood –> Lesions in nodes, lung, liver, spleen, nose, skin

28
Q

Glanders - Supparative Lesions

A

Nodular - neutrophils, fibrin, RBCs

Granulomatous - Necrotic, giant cells

29
Q

Glanders - Active Infection

A

Fever
Nasal discharge
Lymphandenitis
Frequently fatal

30
Q

Glanders - Chronic Infection

A

Fever and resp problems

Skin abscesses

31
Q

Glanders - Diagnosis

A

CFT - main

ELISA, HAI

32
Q

B. Pseudomallei - Malioidosis

A

Pyogranulomatous infection
Reportable
BSL3
Zoonotic

33
Q

Malioidosis - Real Saprophyte

A

Water rich environments
Infections after flooding/heavy rain
Swamps

34
Q

Malioidosis has endosymbiont with

A

Amoeba (reservoir)

35
Q

Malioidosis - Host

A

Wide range - large dissemination
Mammals - sheep, goat mainly
Rodents

36
Q

Malioidosis - Transmission

A

Ingestion
Wound infection
Airborne
Arthropod bites

37
Q

Malioidosis - Virulence

A

Adhesins - flagella
Capsule
T3SS and T4SS

38
Q

Malioidosis - in Dogs

A

Febrile diseases w/ localizing suppurative foci

39
Q

Malioidosis - in Equine

A

Mimics glanders - pseudoglanders

40
Q

Malioidosis - in Cattle

A

Acute to chronic

lungs, joints and uterus

41
Q

Malioidosis - in Sheep

A

Arthritis and lymphadenitis

42
Q

Malioidosis - in Goat

A

Loss of condition, respiratory and CNS disturbances
arthritis
mastitis

43
Q

Malioidosis - in Swine

A 
Loss of condition, resp and CNS disturbances
Arthritis
Mastitis
Abortion
Diarrhea

RUSVM Bacteriology (6 decks)

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