E. Coli Flashcards
Characteristics of E. Coli
Lactose positive
Oxidative negative
Motile Rods
Pathogenicity
Facultative
Obligate pathogenic depending on age of host
O =
O Anitigen 26
H =
Flagella 11
F =
Fimbriae 41
K =
Capsule 60
ETEC
Enterotoxigenic
EHEC
Enterohemorrhagic
VTEC/STEC
Verotoxigenic/Shiga like toxic
EPEC
Enteropathogenic
RPEC
Rabbit pathogenic
APEC
Avian pathogenic
NTEC
Neurotoxigenic (cattle only)
F4
Only in pigs
Mannose resistant
Genes expressed at body temp
F5
Pigs and Bovine
Mannose resistant
Temp dependent
Only in young animals
F6
Neonatal animals
F18
Seen in edema
F41
Pigs and Bovine
Mannose resistant
Labile Toxin (LT)
Heat labile
Decrease Na+ absorption
Increase Cl- and HCO3+ secretion
Withstand 30 min at 60C
STa
Activation of guanylate cyclase system
Decrease Na+
Increase Cl- and HCO3+
STb
Increase prostaglandin E2
EAST1
Similar to STa
Stable Toxin
STa
STb
EAST1
Withstand 15 min at 121C
ETEC in Pigs - Symptoms
Smelly diarrhea
Dehydration
ETEC in Pigs - Target
Neonates - 2-4 wks
ETEC in Pigs - Virulence Factors - Neonate
F 4,5,6,41
STa,b EAST1, LT
ETEC in Pigs - Virulence Factors - Neo-Weaning
F4
LT, STa,b, EAST1
ETEC in Pigs - Epidemiology
Disruption of equilibrium between maternal immunity and infection pressure
Sow - MMA
Piglet - Low birthweight
ETEC in Pigs - Pathogenesis
Ascending from lower bowel –> oral uptake by host –> SI colonization
Requires - adhesion, multiplication, enterotoxins, secondary septicemia
ETEC in Pigs - Diagnosis
Agglutination test
PCR
ETEC in Pigs - Treatment
Hydration
Antimicrobial
ETEC in Pigs - Prevention
Keep infection pressure low and maternal immunity high
Proper Hygiene
Vaccination
Selection of receptor free piglets
VTEC in Pigs - Symptoms
EDEMA - Swollen eyelids, hoarse voice, CNS symptoms
Anorexia