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RUSVM Bacteriology > E. Coli > Flashcards

E. Coli Flashcards

1
Q

Characteristics of E. Coli

A

Lactose positive
Oxidative negative
Motile Rods

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2
Q

Pathogenicity

A

Facultative

Obligate pathogenic depending on age of host

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3
Q

O =

A

O Anitigen 26

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4
Q

H =

A

Flagella 11

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5
Q

F =

A

Fimbriae 41

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6
Q

K =

A

Capsule 60

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7
Q

ETEC

A

Enterotoxigenic

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8
Q

EHEC

A

Enterohemorrhagic

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9
Q

VTEC/STEC

A

Verotoxigenic/Shiga like toxic

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10
Q

EPEC

A

Enteropathogenic

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11
Q

RPEC

A

Rabbit pathogenic

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12
Q

APEC

A

Avian pathogenic

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13
Q

NTEC

A

Neurotoxigenic (cattle only)

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14
Q

F4

A

Only in pigs
Mannose resistant
Genes expressed at body temp

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15
Q

F5

A

Pigs and Bovine
Mannose resistant
Temp dependent
Only in young animals

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16
Q

F6

A

Neonatal animals

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17
Q

F18

A

Seen in edema

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18
Q

F41

A

Pigs and Bovine

Mannose resistant

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19
Q

Labile Toxin (LT)

A

Heat labile
Decrease Na+ absorption
Increase Cl- and HCO3+ secretion
Withstand 30 min at 60C

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20
Q

STa

A

Activation of guanylate cyclase system
Decrease Na+
Increase Cl- and HCO3+

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21
Q

STb

A

Increase prostaglandin E2

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22
Q

EAST1

A

Similar to STa

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23
Q

Stable Toxin

A

STa
STb
EAST1
Withstand 15 min at 121C

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24
Q

ETEC in Pigs - Symptoms

A

Smelly diarrhea

Dehydration

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25
Q

ETEC in Pigs - Target

A

Neonates - 2-4 wks

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26
Q

ETEC in Pigs - Virulence Factors - Neonate

A

F 4,5,6,41

STa,b EAST1, LT

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27
Q

ETEC in Pigs - Virulence Factors - Neo-Weaning

A

F4

LT, STa,b, EAST1

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28
Q

ETEC in Pigs - Epidemiology

A

Disruption of equilibrium between maternal immunity and infection pressure
Sow - MMA
Piglet - Low birthweight

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29
Q

ETEC in Pigs - Pathogenesis

A

Ascending from lower bowel –> oral uptake by host –> SI colonization
Requires - adhesion, multiplication, enterotoxins, secondary septicemia

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30
Q

ETEC in Pigs - Diagnosis

A

Agglutination test

PCR

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31
Q

ETEC in Pigs - Treatment

A

Hydration

Antimicrobial

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32
Q

ETEC in Pigs - Prevention

A

Keep infection pressure low and maternal immunity high
Proper Hygiene
Vaccination
Selection of receptor free piglets

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33
Q

VTEC in Pigs - Symptoms

A

EDEMA - Swollen eyelids, hoarse voice, CNS symptoms

Anorexia

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34
Q

VTEC in Pigs - Virulence

A

F 4,18

St2e

35
Q

VTEC in Pigs - Epidemiology

A

Sow to piglet

Weaning –> stress, changes in intestinal flora, maternal immunity

36
Q

VTEC in Pigs - Pathogenesis

A

Excretion –> infection pressure increases –> contamination of other piglets

37
Q

VTEC in Pigs - Toxin

A

Hyper acute - enterorrhagia
Acute - permeability blood vessels edema
Slower - intravascular coagulation
Endotoxins - Shock and acute mortality

38
Q

VTEC in Pigs - Diagnosis

A

Clinical signs
Pathologic findings
Bacteriology
Virulence factors

39
Q

VTEC in Pigs - Treatment

A

Reduce stress, Feed composition, Flora adaptation

Not yet affected - antimicrobial therapy

40
Q

VTEC in Pigs - Prevention

A

Reduce stress
Feed composition
Adaptation of flora - probiotics, vaccination
Receptor negative animals

41
Q

EPEC in Pigs

A

Rare
Diarrhea at weaning
Diagnosis - Isolation + PCR

42
Q

UTI in Pigs - Predisposing Factors

A 
Hygiene
Water intake
Obstipation
Age
Individual
43
Q

UTI in Pigs - Symptoms

A

Anorexia
Hematuria
Vaginal exudate
General Malaise

44
Q

UTI in Pigs - Diagnosis

A

Bacteriology

45
Q

UTI in Pigs - Treatment

A

Culling

Antibiotics

46
Q

MMA of PPDS in Pigs - Etiology

A

Heredity
Hormonal
Feed
Infectious agents

47
Q

MMA

A

Metritis Mastitis Agalactiae

48
Q

PPDS

A

Post Partum Dysgalactiae Syndrome

49
Q

ETEC in Bovine - Symptoms

A

Diarrhea

Dehydration

50
Q

ETEC in Bovine - Target

A

Less than 3 days old

51
Q

ETEC in Bovine - Virulence

A

F 5,41,17 CS31A

STa,b

52
Q

ETEC in Bovine - Pathogenesis

A

Orap uptake –> XXX intestine –> enterotoxin production –> secondary septicemia
Immunity/infection pressure imbalance

53
Q

ETEC in Bovine - Diagnosis

A

ELISA

Culture + Virulence factor

54
Q

ETEC in Bovine - Therapy

A

Separate sick animals
Provide hydration
Antibiotics
NSAIDs

55
Q

ETEC in Bovine - Prevention

A

Hygiene - separate calves from mothers

Antibiotics

56
Q

EHEC and EPEC (eae) in Bovine - Target

A

1-8 week old

57
Q

EHEC and EPEC in Bovine - Symptoms

A

Mucoid diarrhea +/- blood

Mainly in large intestine

58
Q

EHEC in Bovine Zoonotic?

59
Q

Septicemia in Bovine - Virulence

A

Serum resistance
Iron uptake systems
Capsule
F17, CS31A, CNF1 and CNF2

60
Q

Septicemia in Bovine - Pathogenesis

A

Bacteria PO –> lymph tissue of head, throat, intestine

Umbilical cord –> septicemia colostrum

61
Q

Septicemia in Bovine - Hyperacute

A

Acute mortality

Shock

62
Q

Septicemia in Bovine - Acute

A

General symptoms

Fast mortality

63
Q

Septicemia in Bovine - Slow w/ organ localization

A

Poly arthritis
Pneumonia
Pleuritis
Menigo - encephalitis

64
Q

Septicemia in Bovine - Target

A

Neonates - lack of colostrum

65
Q

Septicemia in Bovine - Therapy

A

NSAID
Antibiotics
Gamma globulins

66
Q

E Coli Cats and Dogs - Enteric

A

Isolation in feces

67
Q

E Coli Dogs - Cystitis

A

Fimbriae - Type 1, F12,134
Specific serotypes
50% alpha-hemolytic

68
Q

E Coli Cats - Cystitis

69
Q

E Coli Cats and Dogs - Pyometra

A

Same strain as cystitis in dogs

70
Q

E. Coli in Poultry

A

Most important bacterial disease in broilers and layers

71
Q

E. Coli in Poultry - Pathogen type

A

Facultative

72
Q

E. Coli in Poultry - Serotypes

A

O1, O2, O78

73
Q

E. Coli in Poultry - Clinical Signs

A

Neonatal - Colibacillosis
Respiratory - colibacillosis and septicemia
Peritonitis

74
Q

E. Coli in Poultry - Pathogenesis Neonatal

A

Yolk rest infection –> sepsis –> death
Yolk rest infection –> Polyserosistis/airsac/pericardia –> growth retardation
Contamination of egg shell
Neonatal contamination
Manipulation of chicks

75
Q

E. Coli in Poultry - Pathogenesis Respiratory Colibacillosis

A

Excretion in feces –> increase infection pressure –> Inhale E. Coli –> resp. disease

76
Q

E. Coli in Poultry - Pathogenesis Peritonitis in Layers

A

Endogenic infection
Start of lay
Chronic form in older animals

77
Q

E. Coli in Poultry - Diagnosis

A

Pathological lesions

Isolation of E. Coli from organs

78
Q

E. Coli in Poultry - Treatment

A

Antibiotics - susceptibility test

79
Q

E. Coli in Poultry - Prevention

A

Improve hygiene
Decrease stress
Temp. Control
Vaccines

80
Q

E. Coli O78, O2 Causes

A

Leads to Scabby hip/necrotic dermatitis in broilers

Prevention - Lower animal density

81
Q

E. Coli in Rabbits - RPEC

A

EPEC (eae)
Diagnosis - symptoms, isolation of subtyping
Antibiotics

82
Q

APEC Virulence Factors

A

Serum Resistance
Capsule
Iron Acquisition
LPS - LipidA

83
Q

ETEC in Pigs - Virulence Factors - Weaning

A

F 4,18

STa,b EAST1

84
Q

EHEC Bovine Neonate Virulence

A

EAE + VT1 or VT2

RUSVM Bacteriology (6 decks)

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