Pseudomonas aeruginosa

Question 1

morphology

Answer 1

heterotrophic, motile, Gram-negative rod-shaped lactose non-fermenting bacterium; oxidase positive

Question 2

virulence

Answer 2

Exotoxin A - ADP ribosylation of EF2 to disrupt translation/protein synthesis

Endotoxin (sepsis)

Biofilm formation for adherence

Attachment mediated via pili and extracellular polysaccharide slime

Hemolytic Phospholipase C Suppresses Neutrophil Respiratory Burst Activity

iron- toxic to endothelium

Quorum sensing system; elaborates virulence through process of cell–cell communication that allows bacteria to share information about cell density and adjust gene expression accordingly

Question 3

Risk factors

Answer 3

diabetes, chemotherapy, cystic fibrosis patient

Question 4

Lab Diagnosis

Answer 4

Culture demonstrates Bluegreen pigmented colonies with sweet grape like fruity odor;

green pigmentation results from production of two water soluble dyes- blue procyanin and yellow fluorescein

Question 5

what does it mean to oxidase postive

Answer 5

posses cytochrome oxidase enzyme which functions as major oxygen reductase in well-aerated cultures

Question 6

produces green pigmentation

Answer 6

blue procyanin (Stimulates IL-8 and C5apeptidase) and yellow fluorescein

Question 7

aerobic or anaerobic

Answer 7

aerobic