Proving DNA Replication is Semi-Conservative Flashcards
1
Q
How is DNA replication semi-conservative?
A
a new DNA molecule is made of one original strand and one new strand
2
Q
How is DNA replication proved to be semi conservative?
A
population cultured in a growth medium containing heavy nitrogen ( 15 N) only.
when centriguued only one heavy bond is observed
cells transferred to a medium with only light nitrogen (14 N)
after one replication the DNA bond was intermediate - twice the thickness
3
Q
What is after two replications?
A
light nitrogen
intermediate and light bonds were observed
proves DNA replication is semi-conservative
4
Q
*****MEMORISE THE DIAGRAM
A
