Protozoa 2 Flashcards
Giardia intestinalis
-host
- dogs, cats, cattle, HUMANs
- Assemblage specific
Giardia intestinalis
- ID
- site of infection
- trophozoites, cysts
- small intestine
Giardia intestinalis
-pathogenesis/lesions
- attach to microvilli of proximal small bowel to feed, occasionally cause duodenal ulcerations
- malabsorption common
- asymtomatic, trophozoites can cause acute focal inflammation
Giardia intestinalis
- CS
- Dx
- none, acute or chronic diarrhea
- fecal smear demonstrates cysts and trophozoites; fecal flotation with zinc sulphate; ELISA
Giardia intestinalis
-Tx/prevention
- no tx are approved in the USA for dogs and cats. Parasiticides & hygiene
- several benzimidazole remedies approved for cattle
- prevent with sanitation & disinfection
Spironucleus (Hexamita)
- hosts
- ID
- Domestic & wild fowl, NOT chickens
- two nuclei, flagellated
- one species infects turkeys, one species infects pigeons
Spironucleus (Hexamita)
-LC
- Same as Giardia
- birds vs mammals
- the large vs small intestine
- infectious catarrhal enteritis
- young birds affected
- droppings from carrier birds
- contamination of shoes or equipment carries fecal material to one location from another
- trophozoite and cyst stages
Spironucleus (Hexamita)
- Site of infection
- Pathogenesis/lesions/CS
- Large intestine
- listlessness, watery or foamy diarrhea, rapid weight loss, severe losses in young birds of 3-5w
Spironucleus (Hexamita)
-dx
history, CS, demonstrate flagellate organisms in intestinal contents
Tritrichomonas foetus
- hosts
- ID
- cattle
- only trophozoite stage
- 3 anterior flagella
- undulating membrane
- pear shaped
Tritrichomonas foetus
-LC
- in urogenital tract
- in the prepuce of bull
- in vagina, cervix and uterus in cows
- transmitted by coitus
Tritrichomonas foetus
- site of infection
- pathogenesis/lesions
- uterus, cervix and vagina in cow
- prepuce in bull
- in bull there may be discharge, but therefore no lesions
- in cow there may be endometritis
Trichomonosis in bulls
- organism survives in microscopic folds of skin that line the bull’s penis and internal sheath
- asymptomatic life time carrier
- older bulls (over 3 years) more likely to become chronically infected than younger bulls
Trichomonosis in cows
- the organism lives in the vagina, cervix, uterus
- short lived, a cow or heifer can become repeatedly infected
- cow may show a subtle, mild, vaginal discharge 1-3w (pyometra)
- inflammatory response leading to early abortion
- repeated breeding leading to wide ranges of gestational ages at pregnancy checks
Trichomonas foetus
-Dx
- PCR; differentiate contaminate trichomonads; also detect dead cells
- culture: slow (days), detect live cells only
Trichomonoas foetus Diagnosis is on a herd basis:
- the bulls go back to work at the end of a long breeding season
- a lot of open cows at the end of a short breeding season
- 10-40% reduction in pregnant cows at pregnancy check time
- a spread out calving season
- three consecutive tests recommended for bulls, once a week for 3 consecutive weeks
Trichomonoas foetus Dx Bulls
- best to test after 2 weeks of sexual rest
- usually culture for up to 7 days
- if even one bull is positive, the whole herd are assumed to have been exposed
Trichomonoas foetus Dx Cows
- Usually clear infection rapidly-often in one season
- test soon after embryo death
- months delay for suggestive hx in a herd
Tritrichomonas foetus
-Tx/prevention
- no approved tx
- prevention is the key
- REPORTABLE
Feline trichomonosis
-risk factors
- age- one yr old or younger
- breed- purebred
- Hx of diarrhea
- co-infection w/ other enteric protozoa
Feline trichomonosis
- transmission
- symptoms
- primary infection is by fecal-oral route
- asymptomatic
- symptomatic cats show diarrhea- cow pie like stools that are gassy and malodorous
Feline trichomonosis
- Dx
- Tx
Direct fecal smear, fecal culturing, PRC
-Ronidazole
Trichomonas spp
- common name
- hosts
- Canker (pigeons)
- Frounce (hawks)
- domestic & wild fowl
Trichomonas
-ID
-trophozoite pear shaped, single nucleus, four flagella and axostyle, similar to Tritrichomonas of cattle