Protozoa Flashcards
monomorphic tryapnosmosis
t. vivax, t. congolense,
pleomorphic trypanosmosis
t. brucei
morphology of trypanosmosis
10-40 μm
large nucleus
kinetoplast
free flagellum
undulating membrane
cyclic transmission example
t. brucei
non cyclic tranmission example
t. evansei
heteroxenous
cyclic
biological vector
homoxenous
non cyclic
mechanical vector
phenotypes of trypanosomosis
trypomastigote
promastigote
epimastigote
amastigote
diseases of trypanosomosis
nagana
surra
dourine
chagas
cause of nagana
t. vivax
t. congolense
t. brucei brucei
vector of nagana
tsetse fly
hosts of nagana
horses
cattle
camel
dog
cat
symptoms of nagana
oedema
lymph node enlargement
splenomegaly
diagnosis of nagana
detection of trypomastigotes from blood and lymph nodes
serology
necropsy
cause of surra
t. brucei evansai
vectors of surra
mechanical - blood sucking flies
biological - vampire bats
hosts of surra
camels
horses
dogs
surra symptoms in horses
paralysis
oedema
surra symptoms in dogs
keratitis
oedema
haemorrhages
cause of dourine
genetic variants of t. brucei evansi and t. brucei equiperdum
clinical signs of dourine
genitals - inflammation and ulceration of mm, oedema, hypopigmentation of skin
skin - dollar spors
nerves - hyperaesthesia, anaesthesia, ascending motor paralysis
hosts of dourine
horse
donkey
cause of chagas
t. cruzi
host of chagas
human
animals
biological vector of chagas
bed bugs
kissing bugs
symptoms of chagas
pseudocycst in myocardium
red inflammed skin
leishmaniosis
l. tropica
l. infantum
l. donovani
l. chagasi
hosts of leishmaniosis
human
dog
cat
vector of leishmaniosis
biological vector - sandfly
forms of leishmaniosis
cutan and visceral forms
cutan leishmanisosis
alopecia
dermatitis
ulceration of the skin
visceral leishmanisosis
fever
lymph node enlargement
spleen
liver
cachexy
anaemia
diarrhoea
ataxia
diagnosis of leishmaniosis
amastigote detection in smear or scrappings
biopsy of lymph node, liver, spleen, marrow or skin
serology
necropsy
hosts of giardia
human
young animals
flagella in giardia
4 pairs
2 nuclei
location in dogs of giardia
duodenum
location in cats of giardia
jejunum, ileum
forms of giardia
trophozoites
cysts
trophozoites of giardia morphology
10-20 μm
pear shaped
symmetrical,
two nuclei – on muscosal surface
trophozoites of giardia
vegetative form
cysts of giardia
infectious form
cysts of giardia morphology
9-15 μm
oval
four nuclei (resistant)
clinical signs of giardia
malabsorption
pancreatic insufficiency -EPI
emaciation
poor growth
mucous, yellow and slimy diarrhoea
loss of appetite
vomitting
abdominal pain
parasitology diagnosis of giardia
faecal smear
flotation
serology
snap test
trichomonosis of cattle
t. foetus
forms of trichomonosis
only trophozoite
trichomonosis with 4 flagella
t. gallinae
t. vaginalis
tetratrichomonas gallinarum stb
trichomonosis with 3 flagella
t. foetus
t. equi stb
trichomonosis with 5 flagella
pentatrichomonas hominis stb
clinical signs of trichomonosis in bulls
inappetance
sometimes inflammation of mm of preputium
urethral discharge
clinical signs of trichomonosis in cows and heifers
vestibulitis
vaginitis
ascending endometritis
pyometra
vaginal discharge
anoestrus
diagnosis of trichomonosis
collecting of vaginal/uretal discharge
serology
PCR
necropsy
necropsy finding of trichomonosis
greyish yellow papules on chorion of the foetus
trichomonosis of bird
t. gallinae
hosts of t. gallinae
pigeons
turkeys
chickens
clinical signs of trichomonosis in birds
visible yellowish pseudomembranes
necrotic lesions in mouth and pharynx
foul odour in mouth
pendulous crop
apathy
diarrhoea
suffocation
cachexy
diagnosis of trichomonosis in birds
smear from lesions
diagnosis of trichomonosis in birds
smear from lesions
necropsy of trichomonosis in birds
yellowish diphtheritic membrane in mouth, pharynx, oesophagus, proventriculus, crop
lesions in liver, heart and air sacs
histomonosis cause
histomonas meleagridis
host of histomonosis
young turkeys
chicens
guinea fowl
pheasant
where is flagellated histomonosis found
caecum tissue
where is amoeboid histomonosis found
tissues
clinical signs of histomonosis
loss of appetites
diarrhoea
yellow faeces
blackhead
what is blackhead of histomonosis
cyanosis of head, comb and wattle
diagnosis of histomonosis
difficult
Detection of flagellate form (10-20(30) μm, round or ovoid with one flagellum) in
caecal lumen
Detection of amoeboid trophozoites in tissues (8-15 μm, round without flagellum)-
pleomorphic
necropsy findings of histomonosis
caecum - yellowish, necrotic, caseous material in lumen, thickened wall
liver - crateriform necrotic foci
in guinea fowl - necrotic lesions in spleen, lung, kidneys
morphology of eimeria
homoxenous
stenoxenous
intracellular
types of eimeria
sporulated oocytes
unsporulated oocytes
structure of eimeria sporulated oocyst
2 sporocysts each containing 2 banana shaped sporozoites
schizont of eimeria size
300μm
merozoite of eimeria size
5-10μm, crescent shape
unspolorulated oocyst
15-50 μm size
ellipsoidal, ovoid or spherical shape
Refractive shell, micropyle (a small pore)
with a polar cap Zygote within the shell
diagnosis of eimeria
mucosal or faecal smear
necropscopy
histological section
7 species of eimeria in chickens
e. tenella
e. necatrix
e. brunetti
e. maxima
e. acervuline
e. mitis
e. praecox
clinical signs of eimeria depends on
speices
amount of ingested sporulated oocysts
virulence of species
resistance of host
clinical signs of eimeria in chickens
anorexia
moribund appearance, lethargy
hypopigmentation
ruffled feathers
soft bloody faeces
sucumb
decreased weight and egg prodcution
diagnosis of eimeria
flotation of unsporulated oocysts in faeces
e. tenella clinical signs
blood in lumen
wall is dilated and thick
epithelial erosion
schizonts and merozoites in caecum
death
e. tenella location
caecum
e. necatrix location
wall of middle third of small intestine
jejunum, ileum
e. necatrix clinical signs
unclotted blood in lumen
pepper and salt appearance
haemorrhage
thickened and swollen wall
e. brunetti clincial signs
haemorrhage
catarrhal exsudate
coagulatie necrosis
pethechiae
ladder like appearance
e. brunetti location
lower small intestine
prox. caecum
colon
rectum
cloaca
e. maxima location
mid small intestine
clinical signs of e. maxima
inflammed
ballooned
thickened mucosa
orange/salmon punk exudates in lumen
e. acervulina location
duodenum
clinical signs of e. acervulina
watery lumen contents
white foci
intestinal geese coccidiosis
e. anseris
e. nocens
coccidiosis of geese
intestinal and renal
clinical signs of e. anseris
anorexia
polydipsia
diarrhoea
unstable gait
types of coccidiosis in geese
intestinal
renal
causes of intestinal coccidiosis in geese
e. anseris
e. nocens
clinical signs of intestinal coccidiosis in geese
anoerexia
polydipsia
weakness
unstable gait
diarrhoea
necropscopy of intestinal coccidiosis in geese
distended middle to lower third of the small intestine
reddish brown fluid in lumen
cause of renal coccidiosis in geese
e. truncate
clinical signs of renal coccidiosis in geese
- weakness,
- anorexia,
- diarrhoea,
- sunken eyes,
- torticollis,
- sometimes paralysis
- Disturbances in balance (vertigo)
- Lying supine
necroscopy of renal coccidiosis in geese
enlarged kidney
greyish/yellow
white foci
coccidiosis of cattle
e. zuernii
e. bovis
pathogen of e. zurenii
schizogony
symptoms beofre oocyst shedding
pathogen of e. bovis
gametogony
symptoms appear at same time as oocyst shedding
clinical signs of cattle coccidiosis
diarrhoea
haemorrhage
mucous or blood in faeces
rough hair coats
sunken eyes
cachexy
tenesmus
rectal prolapse
forms of rabbits coccidiosis
biliary and intestinal
cause of biliary coccidiosis in rabbit
e. stiedai
clinical signs of biliary coccidiosis in rabbit
pu
anorexia
wasting
digestive problems
cirrhosis
jaundice
diagnosis of biliary coccidiosis in rabbit
faeces or flotation
necroscopy of biliary coccidiosis in rabbit
hepatomegaly
greyish yellow nodules on live
pus or caseous filled lesions
histopath of biliary coccidiosis in rabbit
biliary hyperplasia
cholangitis
bile duct distension
cause of intestinal coccidiosis in rabbits
e. intestinalis
e. flavescens
e. irresidula
e. piriformis
e. magna
e. perforans
e. coecicola
when is intestinal coccidiosis in rabbits seen
after weaning at age of 1-2months
clinical signs of intestinal coccidiosis in rabbits - subcinical
no symptoms
clinical signs of intestinal coccidiosis in rabbits
anorexia
increased water consumption
weight loss
emaciation
death
dehydration
diarrhoea
diagnosis of intestinal coccidiosis in rabbits
detection of unsporulated oocyst with flotation
necroscopy of intestinal coccidiosis in rabbits
catarrhal or haemorrhagic enteritis
thickening of mucosa in smal intestine or large
pinhead white nodules in ileum - e. magna
histopath of intestinal coccidiosis in rabbits
desquamation of mm
necorsis of epithelial tissue
cause of coccidiosis of pig
isospora suis
clinical signs of coccidiosis of pig
diarrhoea
never bloody
diagnosis of coccidiosis of pig
faecal smears isnt sensitive
flotation possible after sporulation
autoflourescence
no. of sporozoites in sporocyst of coccidiosis of pig
2 sporocysts containing sporozoites
necroscopy of coccidiosis of pig
lesions in mucosa of small intestines
treatment of coccidiosis of pig
baycox
cause of coccidiosis of dog
isospora canis
isospora ohioensis
isospora burrowsi
clinical signs of coccidiosis of dog
diarrhoea - days in kittens. weeks in puppies
emaciation
anaemia
dehydration
exsiccosis
enteritis
in which isospora is there diarrhoea before detecting oocyst in faeces
l. canis
detection of coccidiosis of dog
flotation
cause of coccidiosis of cat
isospora felis
isospora rivolta
cause of cryptosporidiosis in mammals
c. parvum
c. muris
cause of cryptosporidiosis in birds
c. baileyi
c. meleagridis
size of oocyst in cryptosporidiosis
tiny
5-8um
infection of cryptosporidiosis
po
water/food borne
clinical signs of cryptosporidiosis depend on
parasite
host species
amount oof ingested sporulated oocyst
clinical signs of cryptosporidiosis in mammals
acute diarrhoea
watery pasty faeces
anorexia
fever
dehydration
death
clinical signs of cryptosporidiosis in birds
bursa fabricii atrophy
sneezing, coughing, dyspnoe
diagnosis of cryptosporidiosis
flotation
need microscope
mucosa smear
immunological methods
necroscopy of cryptosporidiosis in mammals
enteral catarrhal in ileum
cellular infiltration of propria
in abomasum in c. muris
necroscopy of cryptosporidiosis in birds
inflammation in airsacs
pneumonia
sinusitis
histo changes in small and large intestines, cloaca, bursa fabricii
cause of toxoplasmosis
toxoplasma gondii
final host of toxoplasmosis
species of felidae
intermediate host of toxoplasmosis
mammals and birds
infection of toxoplasmosis
po
meat contatin xyst
sporulated oocyst
galacgogenically
transplacental
paretnal
clinical signs of toxoplasmosis
infection usually latent without clinical signs
clinical signs of toxoplasmosis in cats
fever
anorexia
ocular signs
encephalitis
enteritis
pneumonia
clinical signs of toxoplasmosis in dog
jaundice
anorexia
diarrhoea
pneumonia
CNS
clinical signs of toxoplasmosis in horses
nervous signs
clinical signs of toxoplasmosis in pregnant ewes
abortion
clinical signs of toxoplasmosis in swine
rarely fever
apathy
diarrhoea
diagnosis of toxoplasmosis
unsporulated oocyst with flotation only in cat faeces
sabin feldman dye
biopsy, csf
tissue culture
pcr
neuroimaging
thallium 201
necroscopy of toxoplasmosis
aborted foetus
yellowish - white necrotic spots in placenta
lymphocytic infiltration of brain
foci and granulomas in liver, spleen, lungs, brain of rabbits
dissemination in young dogs in conjunciton with rubarth’s diseaase, canine distemper
cause of besnoitiosis in cattle
besnoitia besnoiti
intermediate host of besnoitiosis
cattle or wild ru
final host of besnoitiosis
unknown
transmission of besnoitiosis
flies
clinical signs of acute besnoitiosis
anasarca phase
anorexia
fever
swollen ln
rhinitis
dyspnoea
sc oedema
clinical signs of chronic besnoitiosis
scleroderma phase
skin
thickening
wrinkling
alopecia
abortion and sterility
diagnosis of besnoitiosis
cysts in sclera, conjunctiva, mucosa of resp and genitals
biopsy of skin
serology
necroscopy of besnoitiosis
cysts on serosal, mucosal surfaces
cause of sarcocytiosis
s. cruzi
s. calchasi
final host of sarcocytiosis - intestinal sarcosporidiosis
dog
cat
wild car
man
intermediate host of sarcocytiosis - muscle sarcosporidiosis
cattle
sheep
swine
horse
rabbit
clinical signs of sarcocytiosis in carnivores
none
clinical signs of sarcocytiosis in man
signs of acute gastrointestinal malaise
diarrhoae
vomitting
shivering
sweating fever
clinical signs of sarcocytiosis in cattle
anorexia
intermittent fever
emaciation
exophthalmus
anaemia
reduced milk yield
loss of condition
dysponoea
submandibular oedema
enlargement of ln
abortion
dalmeny disease
clinical signs of sarcocytiosis in lambs
anorexia
weakeness
death
abortion
anaemia
clinical signs of sarcocytiosis in swine
biphasal fever
apathy
dysponoea
anaemia
cyanosis
muscle spasm
abortion
diagnosis of sarcocytiosis
in final hosts
sporocysts via flotation
serodiagnosis procedures
antigens
necropsy of sarcocytiosis in sheep
cysts
wheat- pea size greyish yellowish sarcocysts in wall of oesophagus
necropsy of sarcocytiosis in cattle and horse
sarcocysts by naked eye
necropsy of sarcocytiosis in general
haemorrhagic diathesis
enlarged ln
necrotic lesions in liver, kidney and heart
small nectrotic cysts in tongue, heart, oesophagus, skeletal muscle
oedema
fibrosis
cause of hepatozoonosis of dog
hepatozoon canis
vector of hepatozoonosis
rhipicephalus sanquineus - brown dog tick
clinical signs of hepatozoonosis
frequently asymptomatic
fever
lethargy
anorexia
emaciation
anaemia
swelling of ln
lachrymation
nasal discharge
weakness of limbs
muscle pain or stiff gait
bloody diarrhoae
death
diagnosis of hepatozoonosis
blood smear - elliptic gamonts
necroscopy of hepatozoonosis
in all infected organs - inflammatory infiltrates, necroses
cause of babesiosis in equines
b. caballi
cause of babesiosis in cattle
b. divergens
cause of babesiosis in dogs
b. canis
clinical signs of babesiosis
anorexia
anaemia
fever
jaundice
haemoglobinuia
diarrhoea
abortion
death
intermittent fever
pathogenesis of babesiosis
giemsa staining
serodiagnostic methods
necroscopy of babesiosis
anaemia
jaundice
splenomegaly
sub-epicardial, subendocardial haemorrhage on mucosal surface
red and brown red urine in bladder
regeneration of liver and kidney
catarrhal with petechiae in gi mucosa
cause of theileriosis
t. equi
t. annulata
clinical signs of theileriosis
anorexia
anaemia
fever
digestive problems
diarrhoea with mucus and blood
swelling of ln
petechiae haemorrhages on vulva and under tongue
dyspnoea, tachycardia
jaundice, emaciation, intermittent fever
diagnosis of theileriosis
schizonts in bopsy of ln with giemsa staining
merozoites in rbc of blood films with giemsa staining
necroscopy of theileriosis
haemorrhages in serous and mucous membranes
swelling of ln and spleen
ulcer in abomasum and intestine, pulmonary oedema, cachexia
cause of encephalitozoonosis
e. cuniculi
clinical signs of encephalitozoonosis
usually asymptomatic
rarely neurogenic
torticollis or wry neck
paralysis and seizures
iritis, keratis, blindness
diagnosis of encephalitozoonosis
detection of spores in urine
immunological methods
necroscopy of encephalitozoonosis
greyish dents on surface of kidney
morphology of apicomplexa
polar rings
conoid
rhoptries
micronemes
electrondense granules
subpellicular microtubules
micropores
pellicle
Polar ring(s):
thickening of the inner membrane of the pellicle,
organizing subpellicular microtubules running backwards.
Young sporozoites, merozoites have both anterior and posterior p.r.
Conoid:
protrusible, hollow, cone-like structure consisting of
spirally coiled microtubules and situated in the polar ring,
probably providing mechanical help for host cell penetration
Rhoptries:
posteriorly distended, sac-like bodies producing
proteolysins, thus providing enzymatical help for host cell penetration;
also participating in the formation of the parasitophorous vacuole.
Micronemes:
large numbers of small rods producing thrombospondintype proteins for host cell recognition, attachment to it and moving.
Electrondense granules:
located in the mid-region of the cell,
with protein contents initiating metabolic activity in the
parasitophorous vacuole (following exocytosis).
Subpellicular microtubules:
originate from the anterior polar ring,
playing a role in cell motility
Micropore(s):
invagination of the outer layer of the pellicle
(plasmalemma), where the double inner membrane is discontinuous
and thickened. Feeding organelle (for endocytosis).
Pellicle:
outer layer (plasmalemma) continuous, double inner
membrane interrupted at the micropore(s) and polar ring(s).
stages of host cell invasion by apicomplexes
- invasive stage
- exocytosis
- exocytosed microneme material continues expanding on zoite surface
- moving junction seals the pv
development of apicomplexes
asexualy schizigony –> sexual gametegony –> asexual sporogony
coccidia without tissue formation
eimeria
development of eimeria
ingestion of sporulated oocysts
excystation
liberate sporozoites invade epithelial cells of gi mucosa or other organs at the predilection size and become trophozoites
what is schizogony
mitosis
daughter cells rearrange at periphery
sepearation of cytoplasm
the schizont containing merozoites ruptures
merozoites enter other epithelial cells
what is gametogony
the last generation of merozoites differentiate into sexual or hametogonous forms in new host cell
macrogamonts
more female precursor
form with growth and maturation
microgamonts
fewer male precursor
form with further schizogony
prepatent period of eimeria
4-33days - self limiting
prepatent period of chicken coccidiosis
4-6days
sporogony within 12-30days
when are chicken coccidiosis outbreaks most likely
within 3-6weeks of age
location of e. adenoeides
distal ileum, caecum, rectum
location of e. meleagrinitis
jejunum
location of e. gallopavonis
dist ileum
prox caecum
rectum
location of e. dispersa
small intestine
location of e. meleagridis
ileum, dist caecum, rectum
location of e. colchici
ileum
caecum
rectum
location of e. phasiani
ileum
location of e. duodenalis
duodenum
when are pigeons most likely to get coccidiosis
3-4months of age
clinical signs of pigeon coccidiosis
anorexia
diarrhea
dehydration
emaciation
prolonged moult
weakness
death
location of e. anseris
jejunum
ileum
location of e. nocens
ileum