Proteins-amino acids as buffers Flashcards
What’s a buffer
a solution which resists changes in pH when acid/alkali is added to it.
Can amino acids act as a buffers
Yes, When dissolved in water, the amine group and carboxyl group are ionised.This means that the amine group can accept an H+ ion to change from NH2+ to NH3+. The carboxyl group can give up an H+ ion to change from COOH to COO-
Draw the equation of an carboxyl group getting ionised
Draw the equation of an amino group getting ionised
What’s an acid
a substance that produces H ions, H+(aq), when dissolved in water
What’s an alkali/base
accepts H ions(H+)and when added to water, it soaks up the H ions formed by the dissociation of water so that the balance shifts in favor of the hydroxyl ion(OH-)concentration.
How does an amino acid act as a buffer
By accepting H+ ions ,
-At low ph (where there are lots of H+ ions in solution), the amino group (base)will accept H+ ions.
-At high pH (where there are fewer H+ ions in solution), the carboxyl group(acid)will release H+ ions.
How does the carboxyl group act as an acid
It produces H ions H+(aq), when dissolved in water
What does amphoteric mean
able to react both as a base and as an acid.
How does amphoteric nature of amino acids affect protein chains
By accepting and releasing H+ ions amino acids can help regulate changes in Ph = a buffer
Are amino acids amphoteric
Yes