Proteins Flashcards
Glycosylation
Post Translational Modification
Starts at ER andcontinuous in Golgi Apparatus
Eg. Immunoglobulins, Glycoprotein
Protein Stabilisation, Solubility, Orientation, Signalling, Cell Recognision
Disulphide Bridges
Strong Covalent Bonds bw 2 cysteine residues (S-H)
Protein Denaturation Affects
2ry and 3ry Structure
Metalloprotein Functions?
Enzymes
Storage
Signalling
Transport
Globular
Enzymes
Messengers
Transporters
Structural Function in actin and tubulin
Fibrous
Bone Matrices
Muscle Fibre
Tendons
Connective Tissue
Co-operative binding
Binding to one sub-unit
Changes other sub-unit shapes
Increasing their ability to bind to a molecule
Anaemia
Single base change in DNA chain
A switched for T at position 6 of Bchain of haemoglobin
Glutamic acid (hydrophilic aa) is replaced by Valine (hydrophobic aa)
Deoxygenated HbS has abnormally low solubility
At low O2 levels crystals form
RBC change shape
Scurvy
VitC converts Proline to hydroxyproline Lysine to Hydroxylysine Essential in stabilising crosslinks bw chains Weaker Collagen
Osteogenesis Imperfecta
Glycine substituted for large amino acid Protein can no longer fold to form a tight coil Reduced Fibril Interaction Loss of 2ry and 3ry Structure Weakened brittle collage Frequent fractures from a young age
Familial Hypercolesterolemia
Autosomal Dominant Disorder
LDL receptor mutatio
Elevated LDL blood concentration
Cholesterol deposition in skin, tendons, arteries