Proteins 1A Flashcards

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1
Q

What are proteins made from

A

long chains of amino acids

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2
Q

How is a dipeptide formed

A

when two amino acids are joined together

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2
Q

what is the monomer that proteins are made from

A

amino acids

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3
Q

How is a polypeptide formed

A

when more than two amino acids are joined together

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4
Q

What chains are proteins made from

A

one or more polypeptide chains

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5
Q

General structure of an amino acid

A

a carboxyl group, an amine or amino group and a variable group

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6
Q

How many different amino acids are there

A

20

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7
Q

How are amino acids joined together

A

Via condensation reactions to form polypeptides

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8
Q

How do amino acids vary

A

By R groups

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9
Q

What are the bonds between amino acids called

A

peptide bonds

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10
Q

What is the primary structure of a protein

A

the sequence of amino acids in the polypeptide chain

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11
Q

What is the secondary structure of a protein

A

Hydrogen bonds form between the amino acids in the chain. This makes it automatically coil into an alpha helix or fold into a beta pleated sheet

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12
Q

What is the tertiary structure of a protein

A

the coiled or folded chain of amino acids is often folded or coiled further more bonds are formed between different parts of the polypeptide chain including hydrogen bonds and ionic bonds. Disulphide bridges also form whenever two molecules of the amino acid cysteine come close together the sulphur atom in one cysteine bonds to the sulphur atom of another. For proteins made of a single polypeptide chain the tertiary structure forms their final 3D shape.

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13
Q

What is the quaternary structure of a protein

A

Some proteins are made of several polypeptide chains held together by bonds. The quaternary structure is the way these polypeptide chains are assembled together. For proteins which are made up of several polypeptide chains the quaternary structure forms the proteins final structure

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14
Q

Functions of enzymes

A

Enzymes: spherical due to the tight folding of the polypeptide chains. Soluble and often have roles in metabolism and others synthesise large molecules

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15
Q

Functions of antibodies

A

Antibodies: involved in immune response. They’re made up of two light (short) polypeptide chains and two heavy (long) polypeptide chains together. Antibodies also have variable regions the amino acid sequences in these regions vary

16
Q

Functions of structural proteins

A

Physically strong, consist of long polypeptide chains lying parallel to each other with cross links between them. Eg keratin and collagen

17
Q

Functions of transport proteins

A

eg channel proteins, contain hydrophobic and hydrophilic amino acids which cause the protein to fold up and form a channel . These proteins transport molecules and ions across membranes

18
Q

Test for proteins

A

Biuret test:
First add sodium hydroxide solution to the sample to make it alkaline, then add some copper (ii) solution if protein is present then the solution should turn purple if protein is not present then the sample will remain blue