Proteins Flashcards

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1
Q

Proteins always contain

A

-nitrogen
-carbon
-hydrogen
-oxygen

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2
Q

Many proteins may also contain

A

-sulphur and phorphorus

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3
Q

How are proteins made

A

-polymers made out of monomers called amino acids

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4
Q

Chain of amino acids is called

A

polypeptides

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5
Q

how many different amino acids are used to make up proteins

A

20

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6
Q

basic structure of amino acids

A

-an amino group (-NH2) at one end
-carboxyl group at the other end (COOH)
-hydrogen atom
-R group (different in each amino acid)

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7
Q

at ph7 the amino acid has what

A

-positive and negative charge

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7
Q

Name for an amino acid that has a positive and negative group

A

Zwitterion

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8
Q

What are linear sequences of amino acids

A

proteins

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9
Q

How is a peptide bond formed

A

-amino group of one amino acid reacts with the carboxyl group of another and eliminates water (condensation)
-bond between them is the peptide bond
-resulting compound is a dipeptide

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10
Q

Primary structure of a protein

A

-polypeptide chains can have up to 20 amino acids

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11
Q

Primary structure is determined by

A

-base sequence on one strand of the DNA molecule

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12
Q

Secondary structure

A

-shape the polypeptide chain forms as a result of hydrogen bonding
-twisted into 3d shape
-spiral shape - alpha helix
-beta pleated sheet is another form

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13
Q

Keratin proteins

A

-high proportion of alpha helix and the protein fibroin

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14
Q

Silk has a high proportion of

A

-beta pleated sheet

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15
Q

Tertiary structure

A

-the alpha helix of secondary protein folded and twisted to give complex compact 3d structure

16
Q

The tertiary structure is maintained by

A

-hydrogen bonds
-ionic bonds
-disulphide bonds
-hydrophobic interactions

17
Q

what bonds are important in giving globular proteins

A

-hydrogen
-disulphide
-ionic

18
Q

Quaternary structure

A

-some polypeptides are not functional unless combined
-can be associated with non-protein groups and form large, complex molecules like haemoglobin

19
Q

Fibrous proteins

A

-long, thin shape
-insoluble in water so they have structural functions in bones
-polypeptides are in parallel chains or sheets with many cross linkages forming long fibres (keratin-hair)
-strong, tough

20
Q

Collagen is needed in

A

-tendons because they provide strength and the toughness needed

21
Q

A single fibre is normally called a

A

-tropocollagen that consists of 3 identical polypeptide chains twisted around each other like a rope
-3 are linked by hydrogen bonds, stable

22
Q

Globular proteins are

A

-compacted and folded into spherical molecules which makes them insoluble in water

23
Q

Functions of globular proteins

A

-enzyme antibodies
-plasma, proteins and hormones

24
Q

Haemoglobin consists of

A

-four folded polypeptide chains at the centre of each is an iron containing group - haem

25
Q

Test for proteins

A

-few drops of biuret reagent is added
-if protein is present colour change from blue -> purple
-more concentrated the proteins the darker the colour