Proteins Flashcards
to detect the presence of amino acids
purpose of ninhydrin test
oxidative deamination and decarboxylation
principle of ninhydrin test
all positive with proteins except proline
positive result of ninhydrin test
blue-violet solution
positive visible result in ninhydrin test
detects the presence of two or more peptide bonds
purpose of biuret test
complexation of peptide nitrogen with copper
principle of biuret test
sodium hydroxide and copper (II)
reagent of biuret test
positive visible result of biuret test
purple solution
to detect indole rings in amino acids
purpose of hopkins-cole test
principle of hopkins-cole test
condensation and dehydration reaction
purple ring at the junction
positive visible result of hopkins-cole test
to detect arginine and guanidine group
purpose of sakaguchi test
oxidation of guanidine group, condensation with α-naphthol
principle of sakaguchi test
red solution
positive result of sakaguchi
to detect aromatic amino acids
principle of xanthoproteic test
