Protein Trafficking, Secretion & Endocytosis

Question 1

Signal sequence on newly translated protein recruits _________ to be recognized by the Rough ER.

Answer 1

(SRP) Signal Response Protein

Question 2

Cytoplasmic proteins are NEVER _________ because this process is needed for the protein to exit the ER.

Answer 2

Glycosylated

Question 3

A protein is folded by Hsp60 in the RER. What two things must happen for it to exit the ER and be transported to a lysosome?

Answer 3

Glycosylation and Addition of mannose-6-phosphate

Question 4

Glycosylation is important to prevent __________ and is one of the important modifications in the ER to assess ____________.

Answer 4

Degradation, protein folding.

Question 5

(These GTP-dependent proteins) are used to allow a vesicle to find its proper target.

Answer 5

RABs

Question 6

A disease associated with defective peroxisome assembly and no ____________ transported into the peroxisome.

Answer 6

Enzymes, Zellweger Syndrome

Question 7

Describe COP 1, COP 2 and Clathrin coating.

Answer 7

COP I - is a coat that markers vesicles back to the nucleus
“Go back to number ONE.”
COP II - marks vesicles to travel from ER to Golgi
“Where are we taking this TWO?”
Clathrin - coat that heads in any direction
associated with invagination by ligand binding

Question 8

What types of proteins are glycosylated?

Answer 8

Lysosomal enzymes, New proteins modified in R.ER.

Question 9

Select one.

Cytoplasmic proteins are (NEVER/ALWAYS) glycosylated.

Answer 9

NEVER

Question 10

A secretory or lysosomal protein is tagged with ________ to enter the RER to be modified, then ______________ on (this amino acid) _________ to exit the ER. After which, it is further modified in the Golgi for transport or attached to _______ __ ________ if headed to the lysosome.

Answer 10

(SRP) signal recognition particle;
Glycosylated on Asparagine
Mannose-6-Phosphate.