Histology - Cytology

Question 1

What do we use to visualize?

Nuclear Pores

Answer 1

Scanning EM - for a 3D image

Cannot see with LM

Question 2

What do we use to visualize (stain, technique)?

Lysosomes

Answer 2

Stain - Trypan Blue w/ LM

Use immunohistochemistry

Question 3

What do we use to visualize (technique, feature)?

peroxisomes

Answer 3

immunohistochemistry, dark-line crystalline core in center

Question 4

Scenario) Composes spindle fibers in mitosis.
Does it show polarity?
Monomer?

Answer 4

Microtubules
Yes
Tubulin

Question 5

Scenario) Neutrophil uses this to shapeshift and chase after bacterium
What is the biochemical name for this process?

Answer 5

Actin filaments

Chemotaxis

Question 6

Scenario) Found to compose Keratin and calluses
Is it polar?
Filament structure and where was it seen last?

Answer 6

Intermediate Filaments
No
Coiled coils, Leucine zipper

Question 7

What organelles do we see in a cell undergoing constitutive secretion?
Example cell type: __________

Answer 7

Abundant Rough E.R
Few secretory vesicles

(Plasma B cells - secreting ______)

Question 8

What process is a cell undergoing to accumulate proteins in E.R. and store it in vesicles?
When does it release the contents?
Example cell type:__________

Answer 8

Regulated secretion;
Triggers when Signal binds to cell receptors

(Liver cells)

Question 9

A plasma cell or Acinar cell would have an abundance of this organelle.

Answer 9

Rough E.R.

Question 10

A muscle cell or fat cell would have a lot of this type of organelle.

What does this organelle store?

Answer 10

Smooth E.R.

Calcium and synthesized fats

Question 11

What are the distinctive features of mitochondria in these cell types?
Muscle cell: __________
Steroid-producing cell: ___________

Answer 11

3x as many stacked cristae than normal,

Fewer Tubular cristae

Question 12

A random leukocyte rolls in a small blood vessel minding its own business and all. All of a sudden it hits a few speed bumps called ___________. Suddenly it stops in its tracks, poised for action, by the binding of __________. This triggers the WBC to squeeze through a crowd of epithelial cells via ___________ ______________. The whole process is known as ______________ _____________.

Answer 12

Selectins, Integrins,
Transendothelial Migration,
Leukocyte Extravasation