Histology - Blood And ConnTissue

Question 1

Macrophage Features

Answer 1

Many ugly inclusions from pathogens/dust
Lysosomes stained by w/ trypan blue
Clear Foamy cytoplasm

Question 2

Mast Cell Features

Answer 2

Stain pink in LM
Many dark granules (histamine)
Lighter nucleus in comparison
Also stain with toluidine blue

Question 3

Plasma B Cell Features

Answer 3

Big soccer ball nucleus,
Not so clear cytoplasm,
Negative Golgi stain compared to..
Extensive Rough ER

Question 4

Brown Fat Features

Answer 4

Many clear inclusions, more heterogenous
brown periphery staining
Highly vascular, many mitochondria

Question 5

Lymphocyte Features

Answer 5

Small leukocyte, Large nucleus relative to size. LM - Seen as very small dots
EM - have a LARGE nucleus and little cytoplasm

Question 6

Eusinophil Features

Also describe lysosomes

Answer 6

Stain pink/red (compared to basophils)
2-5 Lobes of a segmented nucleus
Looks like a HAMBURGA!
Lysosomes look like a football 
(similar to peroxisome crystalline core)

Question 7

Neutrophil Features

Answer 7

1 Cute non-segmented nucleus

many Granules

Question 8

Collagen Fiber

Answer 8

3 helical collagen strands

LM - Fuzzy pinkish fibers

Question 9

Elastin Fiber

Answer 9

LM - sharper dark fibers with distinct branch pattern

Seen with special orcein stain

Question 10

Reticular Fibers

Answer 10

Collagen 3, Brownish color with silver staining