protein targeting and export 5 Flashcards
vesicles have coats of proteins which are
COP
what do COPs do
reshape membrane to curve/bud determines size/shape of vesicle concentrate in vesicle provides selectivity for cargo determine final destination
how do COPs differ
type of vesicle
what else do they contain
GTP binding protein
V snare
once made and budded vesicles need to be
uncoated to expose the V-snare
v snare to
t snare
clathrin coated vesicles go from what to what
trans golgi network TGN to
endosome and plasma
COP 1 go from what to what
golgi to
ER
cop 2 goes from what to what
ER to
golgi
clathrin subunits are made of what
3 large and
3 small polypeptides
which forms triskelions in the TGN
assembly of clathrin coat
cargo binds to mannose-6-p receptor (selective)
also binds adaptin, which binds triskelions
coat forms
formation and budding assisted by dynamin (GTP)
coat dissociates immediately after
what does dynamin form
forms a helical ring around the neck of the bud and recruits other proteins to help bring the 2 lipids together
Ts mutants of dynamin does what
halts its process and allows visualization of the budding
COP 2 what is it made of
5 protein subunits and
GTPase sar 1
what does COP 2 recruit
enzymes for golgi processing (glycosyltransferase)
docking and fusion proteins
integral proteins that bind to specific cargo
