protein structure Flashcards
protein analysis: explain the principles of electrophoresis, and why proteins with single mutations may be separated by electrophoresis depending on the individual mutations; explain the effects that post-translational modifications may have on protein analysis
1
Q
how are proteins or amino acids analysed
A
electrophoresis
2
Q
what allow mutated amino acids to be detected
A
lower RMM or glycosylation
3
Q
what effect would a mutation creating cysteine (e.g. osteogenesis imperfecta) on electrophoresis
A
cysteine has a greater RMM so travels slower
