Protein quantification and detection Flashcards
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Is Western blot qualititative or quantitative?
Qualitative
Is ELISA qualitative or quantitative?
Quantitative
intensity of the signal is directly correlated to the amount of captured anitgen (spectrophotometer)
PCR does what?
I want to amplify DNA in a copy/paste
A young child of Mediterranean parents was brought to the pediatrician due to lethargy, tiredness, and pallor. Blood analysis revealed a microcytic anemia, although iron levels were normal (see image). What test should be run to determine that the child has a variant of thalassemia?
A. PCR of the gene forg-globinin the child
B. PCR of the gene forRNA polymerase
C. Western blot of snurps in the child
D. Western blot of TFIID
E. Western blotting of the peptide chains in hemoglobin
E. Thlassemia changes the subunits of hemoglobin, which could be ID’d in a western blot
When to use immunohistochemistry?
tissue samples to dye and identify cancerous/ problematic cells
When to use ELISA?
Time constraint, quantitative result is required, but can also be used to give a qualititative result.
IHC is still a potential diagnostic tool for various cancer types because we can use specific markers that will show us a specific type of cancer.
Each marker will tell us where a tumor is coming from, and what type it is. Vimentin marker would be used to determine a cancer cell is a sarcoma
Look at definition of creatine level to know that kidney is doing fine.
Why should we use Western Blot?
Why use immunoprecipitation?
When should we use surface plasmon resonance?
When shoud we use EMSA? (Electrophoretic Mobility Shift Assay)
When to use Multiplexing?
When to use Microarrays?
