Protein Electrophoresis Flashcards
1
Q
How can gel electrophoresis separate proteins?
A
Using their charge and/or size/shape
2
Q
How does gel electrophoresis separate proteins?
A
It uses current flowing through a buffer to separate proteins. The gel acts as a sieve, separating the proteins.
3
Q
What is one form of protein electrophoresis ?
A
SDS-PAGE
4
Q
What happens during SDS-PAGE?
A
The proteins are denatured and given a uniform negative charge; this means that the proteins can be separated based on their size as they migrate towards the positive electrode. Small proteins travel further through the gel than large proteins
5
Q
What is different if you use a native gel?
A
The protein is not denatured before hand. This allows proteins to be analysed in their folded state
